2ffl: Difference between revisions
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|PDB= 2ffl |SIZE=350|CAPTION= <scene name='initialview01'>2ffl</scene>, resolution 3.33Å | |PDB= 2ffl |SIZE=350|CAPTION= <scene name='initialview01'>2ffl</scene>, resolution 3.33Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=MN:MANGANESE (II) ION'>MN</scene> | |LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ffl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ffl OCA], [http://www.ebi.ac.uk/pdbsum/2ffl PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ffl RCSB]</span> | |||
}} | }} | ||
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[[Category: Doudna, J A.]] | [[Category: Doudna, J A.]] | ||
[[Category: MacRae, I J.]] | [[Category: MacRae, I J.]] | ||
[[Category: | [[Category: paz domain]] | ||
[[Category: rna interference | [[Category: rna interference]] | ||
[[Category: rnai]] | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:02:09 2008'' | ||
Revision as of 03:02, 31 March 2008
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| , resolution 3.33Å | |||||||
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| Ligands: | |||||||
| Activity: | Ribonuclease III, with EC number 3.1.26.3 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Dicer from Giardia intestinalis
OverviewOverview
The specialized ribonuclease Dicer initiates RNA interference by cleaving double-stranded RNA (dsRNA) substrates into small fragments about 25 nucleotides in length. In the crystal structure of an intact Dicer enzyme, the PAZ domain, a module that binds the end of dsRNA, is separated from the two catalytic ribonuclease III (RNase III) domains by a flat, positively charged surface. The 65 angstrom distance between the PAZ and RNase III domains matches the length spanned by 25 base pairs of RNA. Thus, Dicer itself is a molecular ruler that recognizes dsRNA and cleaves a specified distance from the helical end.
About this StructureAbout this Structure
2FFL is a Single protein structure of sequence from Giardia intestinalis. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis for double-stranded RNA processing by Dicer., Macrae IJ, Zhou K, Li F, Repic A, Brooks AN, Cande WZ, Adams PD, Doudna JA, Science. 2006 Jan 13;311(5758):195-8. PMID:16410517
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