2f6a: Difference between revisions
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|PDB= 2f6a |SIZE=350|CAPTION= <scene name='initialview01'>2f6a</scene>, resolution 3.29Å | |PDB= 2f6a |SIZE=350|CAPTION= <scene name='initialview01'>2f6a</scene>, resolution 3.29Å | ||
|SITE= | |SITE= | ||
|LIGAND= | |LIGAND= <scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= cna ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus]) | |GENE= cna ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus]) | ||
|DOMAIN= | |||
|RELATEDENTRY=[[2f68|2F68]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f6a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f6a OCA], [http://www.ebi.ac.uk/pdbsum/2f6a PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2f6a RCSB]</span> | |||
}} | }} | ||
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[[Category: mscramm]] | [[Category: mscramm]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:58:31 2008'' | ||
Revision as of 02:58, 31 March 2008
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| , resolution 3.29Å | |||||||
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| Ligands: | |||||||
| Gene: | cna (Staphylococcus aureus) | ||||||
| Related: | 2F68
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Collagen Adhesin and Collagen Complex Structure
OverviewOverview
The structural basis for the association of eukaryotic and prokaryotic protein receptors and their triple-helical collagen ligand remains poorly understood. Here, we present the crystal structures of a high affinity subsegment of the Staphylococcus aureus collagen-binding CNA as an apo-protein and in complex with a synthetic collagen-like triple helical peptide. The apo-protein structure is composed of two subdomains (N1 and N2), each adopting a variant IgG-fold, and a long linker that connects N1 and N2. The structure is stabilized by hydrophobic inter-domain interactions and by the N2 C-terminal extension that complements a beta-sheet on N1. In the ligand complex, the collagen-like peptide penetrates through a spherical hole formed by the two subdomains and the N1-N2 linker. Based on these two structures we propose a dynamic, multistep binding model, called the 'Collagen Hug' that is uniquely designed to allow multidomain collagen binding proteins to bind their extended rope-like ligand.
About this StructureAbout this Structure
2F6A is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.
ReferenceReference
A 'Collagen Hug' model for Staphylococcus aureus CNA binding to collagen., Zong Y, Xu Y, Liang X, Keene DR, Hook A, Gurusiddappa S, Hook M, Narayana SV, EMBO J. 2005 Dec 21;24(24):4224-36. Epub 2005 Dec 15. PMID:16362049
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