2c7m: Difference between revisions
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|PDB= 2c7m |SIZE=350|CAPTION= <scene name='initialview01'>2c7m</scene>, resolution 2.40Å | |PDB= 2c7m |SIZE=350|CAPTION= <scene name='initialview01'>2c7m</scene>, resolution 2.40Å | ||
|SITE= <scene name='pdbsite=AC1:Zn+Binding+Site+For+Chain+A'>AC1</scene> | |SITE= <scene name='pdbsite=AC1:Zn+Binding+Site+For+Chain+A'>AC1</scene> | ||
|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2c7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c7m OCA], [http://www.ebi.ac.uk/pdbsum/2c7m PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2c7m RCSB]</span> | |||
}} | }} | ||
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[[Category: Polo, S.]] | [[Category: Polo, S.]] | ||
[[Category: Schneider, T R.]] | [[Category: Schneider, T R.]] | ||
[[Category: endocytosis]] | [[Category: endocytosis]] | ||
[[Category: nuclear protein]] | [[Category: nuclear protein]] | ||
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[[Category: ubl conjugation]] | [[Category: ubl conjugation]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:17:32 2008'' | ||
Revision as of 02:17, 31 March 2008
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| , resolution 2.40Å | |||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
HUMAN RABEX-5 RESIDUES 1-74 IN COMPLEX WITH UBIQUITIN
OverviewOverview
The interaction between ubiquitinated proteins and intracellular proteins harboring ubiquitin binding domains (UBDs) is critical to a multitude of cellular processes. Here, we report that Rabex-5, a guanine nucleotide exchange factor for Rab5, binds to Ub through two independent UBDs. These UBDs determine a number of properties of Rabex-5, including its coupled monoubiquitination and interaction in vivo with ubiquitinated EGFRs. Structural and biochemical characterization of the UBDs of Rabex-5 revealed that one of them (MIU, motif interacting with ubiquitin) binds to Ub with modes superimposable to those of the UIM (ubiquitin-interacting motif):Ub interaction, although in the opposite orientation. The other UBD, RUZ (Rabex-5 ubiquitin binding zinc finger) binds to a surface of Ub centered on Asp58(Ub) and distinct from the "canonical" Ile44(Ub)-based surface. The two binding surfaces allow Ub to interact simultaneously with different UBDs, thus opening new perspectives in Ub-mediated signaling.
About this StructureAbout this Structure
2C7M is a Protein complex structure of sequences from Bos taurus and Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of the ubiquitin binding domains of rabex-5 reveals two modes of interaction with ubiquitin., Penengo L, Mapelli M, Murachelli AG, Confalonieri S, Magri L, Musacchio A, Di Fiore PP, Polo S, Schneider TR, Cell. 2006 Mar 24;124(6):1183-95. Epub 2006 Feb 23. PMID:16499958
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