2bra: Difference between revisions
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|PDB= 2bra |SIZE=350|CAPTION= <scene name='initialview01'>2bra</scene>, resolution 2.00Å | |PDB= 2bra |SIZE=350|CAPTION= <scene name='initialview01'>2bra</scene>, resolution 2.00Å | ||
|SITE= <scene name='pdbsite=AC1:Cl+Binding+Site+For+Chain+B'>AC1</scene> | |SITE= <scene name='pdbsite=AC1:Cl+Binding+Site+For+Chain+B'>AC1</scene> | ||
|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene> | |LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bra FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bra OCA], [http://www.ebi.ac.uk/pdbsum/2bra PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2bra RCSB]</span> | |||
}} | }} | ||
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[[Category: Gabelli, S B.]] | [[Category: Gabelli, S B.]] | ||
[[Category: Nadella, M.]] | [[Category: Nadella, M.]] | ||
[[Category: axon guidance]] | [[Category: axon guidance]] | ||
[[Category: coiled coil]] | [[Category: coiled coil]] | ||
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[[Category: zinc]] | [[Category: zinc]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:10:39 2008'' | ||
Revision as of 02:10, 31 March 2008
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| , resolution 2.00Å | |||||||
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| Ligands: | , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF N-TERMINAL FAD BINDING MOTIF OF MOUSE MICAL
OverviewOverview
During development, neurons are guided to their targets by short- and long-range attractive and repulsive cues. MICAL, a large multidomain protein, is required for the combined action of semaphorins and plexins in axon guidance. Here, we present the structure of the N-terminal region of MICAL (MICAL(fd)) determined by x-ray diffraction to 2.0 A resolution. The structure shows that MICAL(fd) is an FAD-containing module structurally similar to aromatic hydroxylases and amine oxidases. In addition, we present biochemical data that show that MICAL(fd) is a flavoenzyme that in the presence of NADPH reduces molecular oxygen to H(2)O(2) (K(m,NAPDH) = 222 microM; k(cat) = 77 sec(-1)), a molecule with known signaling properties. We propose that the H(2)O(2) produced by this reaction may be one of the signaling molecules involved in axon guidance by MICAL.
About this StructureAbout this Structure
2BRA is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
ReferenceReference
Structure and activity of the axon guidance protein MICAL., Nadella M, Bianchet MA, Gabelli SB, Barrila J, Amzel LM, Proc Natl Acad Sci U S A. 2005 Nov 15;102(46):16830-5. Epub 2005 Nov 7. PMID:16275926
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