1z3h: Difference between revisions
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|PDB= 1z3h |SIZE=350|CAPTION= <scene name='initialview01'>1z3h</scene>, resolution 3.100Å | |PDB= 1z3h |SIZE=350|CAPTION= <scene name='initialview01'>1z3h</scene>, resolution 3.100Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= Cse1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | |GENE= Cse1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1wa5|1wa5]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z3h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z3h OCA], [http://www.ebi.ac.uk/pdbsum/1z3h PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1z3h RCSB]</span> | |||
}} | }} | ||
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[[Category: Lindner, D.]] | [[Category: Lindner, D.]] | ||
[[Category: Schlenstedt, G.]] | [[Category: Schlenstedt, G.]] | ||
[[Category: cse1]] | [[Category: cse1]] | ||
[[Category: exportin]] | [[Category: exportin]] | ||
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[[Category: nuclear transport]] | [[Category: nuclear transport]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:28:30 2008'' | ||
Revision as of 01:28, 31 March 2008
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| , resolution 3.100Å | |||||||
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| Ligands: | |||||||
| Gene: | Cse1 (Saccharomyces cerevisiae) | ||||||
| Related: | 1wa5
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The exportin Cse1 in its cargo-free, cytoplasmic state
OverviewOverview
Cse1 mediates nuclear export of importin alpha, the nuclear localization signal (NLS) import adaptor. We report the 3.1 A resolution structure of cargo-free Cse1, representing this HEAT repeat protein in its cytosolic state. Cse1 is compact, consisting of N- and C-terminal arches that interact to form a ring. Comparison with the structure of cargo-bound Cse1 shows a major conformational change leading to opening of the structure upon cargo binding. The largest structural changes occur within a hinge region centered at HEAT repeat 8. This repeat contains a conserved insertion that connects the RanGTP and importin alpha contact sites and that is essential for binding. In the cargo-free state, the RanGTP binding sites are occluded and the importin alpha sites are distorted. Mutations that destabilize the N- to C-terminal interaction uncouple importin alpha and Ran binding, suggesting that the closed conformation prevents association with importin alpha.
About this StructureAbout this Structure
1Z3H is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
ReferenceReference
The structure of the nuclear export receptor Cse1 in its cytosolic state reveals a closed conformation incompatible with cargo binding., Cook A, Fernandez E, Lindner D, Ebert J, Schlenstedt G, Conti E, Mol Cell. 2005 Apr 29;18(3):355-67. PMID:15866177
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