3zym: Difference between revisions
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==STRUCTURE OF CALM (PICALM) IN COMPLEX WITH VAMP8== | ==STRUCTURE OF CALM (PICALM) IN COMPLEX WITH VAMP8== | ||
<StructureSection load='3zym' size='340' side='right' caption='[[3zym]], [[Resolution|resolution]] 2.03Å' scene=''> | <StructureSection load='3zym' size='340' side='right' caption='[[3zym]], [[Resolution|resolution]] 2.03Å' scene=''> | ||
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3zyl|3zyl]], [[1hg2|1hg2]], [[1hg5|1hg5]], [[1hfa|1hfa]], [[3zyk|3zyk]], [[1hf8|1hf8]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3zyl|3zyl]], [[1hg2|1hg2]], [[1hg5|1hg5]], [[1hfa|1hfa]], [[3zyk|3zyk]], [[1hf8|1hf8]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zym FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zym OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zym RCSB], [http://www.ebi.ac.uk/pdbsum/3zym PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zym FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zym OCA], [http://pdbe.org/3zym PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zym RCSB], [http://www.ebi.ac.uk/pdbsum/3zym PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zym ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 3zym" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 22:44, 4 August 2016
STRUCTURE OF CALM (PICALM) IN COMPLEX WITH VAMP8STRUCTURE OF CALM (PICALM) IN COMPLEX WITH VAMP8
Structural highlights
Function[PICA_RAT] Assembly protein recruiting clathrin and adaptor protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction (By similarity).[UniProtKB:Q13492] Publication Abstract from PubMedSNAREs provide a large part of the specificity and energy needed for membrane fusion and, to do so, must be localized to their correct membranes. Here, we show that the R-SNAREs VAMP8, VAMP3, and VAMP2, which cycle between the plasma membrane and endosomes, bind directly to the ubiquitously expressed, PtdIns4,5P(2)-binding, endocytic clathrin adaptor CALM/PICALM. X-ray crystallography shows that the N-terminal halves of their SNARE motifs bind the CALM(ANTH) domain as helices in a manner that mimics SNARE complex formation. Mutation of residues in the CALM:SNARE interface inhibits binding in vitro and prevents R-SNARE endocytosis in vivo. Thus, CALM:R-SNARE interactions ensure that R-SNAREs, required for the fusion of endocytic clathrin-coated vesicles with endosomes and also for subsequent postendosomal trafficking, are sorted into endocytic vesicles. CALM's role in directing the endocytosis of small R-SNAREs may provide insight into the association of CALM/PICALM mutations with growth retardation, cognitive defects, and Alzheimer's disease. The Molecular Basis for the Endocytosis of Small R-SNAREs by the Clathrin Adaptor CALM.,Miller SE, Sahlender DA, Graham SC, Honing S, Robinson MS, Peden AA, Owen DJ Cell. 2011 Nov 23;147(5):1118-31. PMID:22118466[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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