4r3s: Difference between revisions
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''' | ==Crystal Structure of anti-MSP2 Fv fragment (mAb6D8)in complex with MSP2 11-23== | ||
<StructureSection load='4r3s' size='340' side='right' caption='[[4r3s]], [[Resolution|resolution]] 1.70Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4r3s]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R3S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R3S FirstGlance]. <br> | |||
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4qxt|4qxt]], [[4qyo|4qyo]], [[4qy8|4qy8]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r3s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r3s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4r3s RCSB], [http://www.ebi.ac.uk/pdbsum/4r3s PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Merozoite surface protein 2 (MSP2) is an intrinsically disordered, membrane-anchored antigen of the malaria parasite Plasmodium falciparum. MSP2 can elicit a protective, albeit strain-specific, antibody response in humans. Antibodies are generated to the conserved N- and C-terminal regions but many of these react poorly with the native antigen on the parasite surface. Here we demonstrate that recognition of a conserved N-terminal epitope by mAb 6D8 is incompatible with the membrane-bound conformation of that region, suggesting a mechanism by which native MSP2 escapes antibody recognition. Furthermore, crystal structures and NMR spectroscopy identify transient, strain-specific interactions between the 6D8 antibody and regions of MSP2 beyond the conserved epitope. These interactions account for the differential affinity of 6D8 for the two allelic families of MSP2, even though 6D8 binds to a fully conserved epitope. These results highlight unappreciated mechanisms that may modulate the specificity and efficacy of immune responses towards disordered antigens. | |||
Structural basis for epitope masking and strain specificity of a conserved epitope in an intrinsically disordered malaria vaccine candidate.,Morales RA, MacRaild CA, Seow J, Krishnarjuna B, Drinkwater N, Rouet R, Anders RF, Christ D, McGowan S, Norton RS Sci Rep. 2015 May 12;5:10103. doi: 10.1038/srep10103. PMID:25965408<ref>PMID:25965408</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
[[Category: | __TOC__ | ||
</StructureSection> | |||
[[Category: Anders, R F]] | |||
[[Category: Bankala, K]] | [[Category: Bankala, K]] | ||
[[Category: Christ, D]] | |||
[[Category: Drinkwater, N]] | |||
[[Category: MacRaild, C A]] | |||
[[Category: McGowan, S]] | |||
[[Category: Morales, R A.V]] | |||
[[Category: Norton, R S]] | |||
[[Category: Rouet, R]] | [[Category: Rouet, R]] | ||
[[Category: Seow, J]] | [[Category: Seow, J]] | ||
[[Category: | [[Category: Immune system]] | ||
[[Category: | [[Category: Immunoglobulin fold]] | ||
[[Category: | [[Category: N-terminal msp2]] | ||
[[Category: | [[Category: Unstructured antigen]] | ||