1ww7: Difference between revisions
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|PDB= 1ww7 |SIZE=350|CAPTION= <scene name='initialview01'>1ww7</scene>, resolution 1.90Å | |PDB= 1ww7 |SIZE=350|CAPTION= <scene name='initialview01'>1ww7</scene>, resolution 1.90Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1ww4|1WW4]], [[1ww5|1WW5]], [[1ww6|1WW6]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ww7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ww7 OCA], [http://www.ebi.ac.uk/pdbsum/1ww7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ww7 RCSB]</span> | |||
}} | }} | ||
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[[Category: Yagi, F.]] | [[Category: Yagi, F.]] | ||
[[Category: Yoon, H J.]] | [[Category: Yoon, H J.]] | ||
[[Category: agrocybe cylindracea galectin]] | [[Category: agrocybe cylindracea galectin]] | ||
[[Category: carbohydrate recognition domain]] | [[Category: carbohydrate recognition domain,x-ray crystallographic analysis]] | ||
[[Category: fungal galectin]] | [[Category: fungal galectin]] | ||
[[Category: sulfate ion]] | [[Category: sulfate ion]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:41:49 2008'' | ||
Revision as of 00:41, 31 March 2008
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| , resolution 1.90Å | |||||||
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| Ligands: | |||||||
| Related: | 1WW4, 1WW5, 1WW6
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Agrocybe cylindracea galectin (Ligand-free)
OverviewOverview
Galectin from an edible fungus Agrocybe cylindracea (ACG) has a strong preference for N-acetylneuraminyl lactose (NeuAcalpha2-3lactose). The sugar recognition mechanism of ACG was explored by the X-ray crystallographic analyses of ligand-free ACG, and its complex with lactose, 3'-sulfonyl lactose and NeuAcalpha2-3lactose. The refined structure shows that ACG is a "proto"-type galectin composed of a beta-sandwich of two antiparallel sheets, each with six strands, in contrast to the five and six strands in animal galectins. ACG dimer in solution was classified as being among the "layer"-type. The carbohydrate recognition domain (CRD) of this galectin is common to those of animal galectins, except for substitution of one residue, Ala64, which corresponds to Asn46 in human galectin 1. A five-residue insertion in ACG at positions 42-46 involving Ser44 and Asn46 modified the architecture of the sugar binding site that contributes sialic acid specificity. Furthermore, it was found that the binding of a sulfate ion near the CRD in the ligand-free form led to a change in the conformation of the loop region caused by main-chain cis/trans transition between Ser44 and Pro45.
About this StructureAbout this Structure
1WW7 is a Protein complex structure of sequences from Agrocybe cylindracea. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis of a fungal galectin from Agrocybe cylindracea for recognizing sialoconjugate., Ban M, Yoon HJ, Demirkan E, Utsumi S, Mikami B, Yagi F, J Mol Biol. 2005 Aug 26;351(4):695-706. PMID:16051274
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