1waf: Difference between revisions
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|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=GMP:GUANOSINE'>GMP</scene> | |LIGAND= <scene name='pdbligand=GMP:GUANOSINE'>GMP</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span> | ||
|GENE= POL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | |GENE= POL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=12353 Enterobacteria phage RB69]) | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1waf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1waf OCA], [http://www.ebi.ac.uk/pdbsum/1waf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1waf RCSB]</span> | |||
}} | }} | ||
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==About this Structure== | ==About this Structure== | ||
1WAF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | 1WAF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_rb69 Enterobacteria phage rb69]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAF OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of a pol alpha family replication DNA polymerase from bacteriophage RB69., Wang J, Sattar AK, Wang CC, Karam JD, Konigsberg WH, Steitz TA, Cell. 1997 Jun 27;89(7):1087-99. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9215631 9215631] | Crystal structure of a pol alpha family replication DNA polymerase from bacteriophage RB69., Wang J, Sattar AK, Wang CC, Karam JD, Konigsberg WH, Steitz TA, Cell. 1997 Jun 27;89(7):1087-99. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9215631 9215631] | ||
[[Category: DNA-directed DNA polymerase]] | [[Category: DNA-directed DNA polymerase]] | ||
[[Category: Enterobacteria phage | [[Category: Enterobacteria phage rb69]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Karam, J D.]] | [[Category: Karam, J D.]] | ||
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[[Category: Wang, C C.]] | [[Category: Wang, C C.]] | ||
[[Category: Wang, J.]] | [[Category: Wang, J.]] | ||
[[Category: nucleotidyltransferase]] | [[Category: nucleotidyltransferase]] | ||
[[Category: rb69 dna polymerase (gp43)]] | [[Category: rb69 dna polymerase (gp43)]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:33:30 2008'' | ||
Revision as of 00:33, 31 March 2008
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| , resolution 3.2Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | POL (Enterobacteria phage RB69) | ||||||
| Activity: | DNA-directed DNA polymerase, with EC number 2.7.7.7 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
DNA POLYMERASE FROM BACTERIOPHAGE RB69
OverviewOverview
The 2.8 A resolution crystal structure of the bacteriophage RB69 gp43, a member of the eukaryotic pol alpha family of replicative DNA polymerases, shares some similarities with other polymerases but shows many differences. Although its palm domain has the same topology as other polymerases, except rat DNA polymerase beta, one of the three carboxylates required for nucleotidyl transfer is located on a different beta strand. The structures of the fingers and thumb domains are unrelated to all other known polymerase structures. The editing 3'-5' exonuclease domain of gp43 is homologous to that of E. coli DNA polymerase I but lies on the opposite side of the polymerase active site. An extended structure-based alignment of eukaryotic DNA polymerase sequences provides structural insights that should be applicable to most eukaryotic DNA polymerases.
About this StructureAbout this Structure
1WAF is a Single protein structure of sequence from Enterobacteria phage rb69. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of a pol alpha family replication DNA polymerase from bacteriophage RB69., Wang J, Sattar AK, Wang CC, Karam JD, Konigsberg WH, Steitz TA, Cell. 1997 Jun 27;89(7):1087-99. PMID:9215631
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