3wdf: Difference between revisions
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==Staphylococcus aureus UDG== | ==Staphylococcus aureus UDG== | ||
<StructureSection load='3wdf' size='340' side='right' caption='[[3wdf]], [[Resolution|resolution]] 1.48Å' scene=''> | <StructureSection load='3wdf' size='340' side='right' caption='[[3wdf]], [[Resolution|resolution]] 1.48Å' scene=''> | ||
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ung, SAR0586 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=282458 STAAR])</td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ung, SAR0586 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=282458 STAAR])</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Uracil-DNA_glycosylase Uracil-DNA glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.27 3.2.2.27] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Uracil-DNA_glycosylase Uracil-DNA glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.27 3.2.2.27] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wdf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wdf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3wdf RCSB], [http://www.ebi.ac.uk/pdbsum/3wdf PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wdf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wdf OCA], [http://pdbe.org/3wdf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wdf RCSB], [http://www.ebi.ac.uk/pdbsum/3wdf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wdf ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 3wdf" style="background-color:#fffaf0;"></div> | |||
==See Also== | ==See Also== | ||
*[[DNA glycosylase|DNA glycosylase]] | |||
*[[Uracil-DNA glycosylase|Uracil-DNA glycosylase]] | *[[Uracil-DNA glycosylase|Uracil-DNA glycosylase]] | ||
== References == | == References == | ||
Revision as of 13:16, 4 August 2016
Staphylococcus aureus UDGStaphylococcus aureus UDG
Structural highlights
Function[UNG_STAAR] Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine (By similarity). Publication Abstract from PubMedDNA mimic proteins are unique factors that control the DNA binding activity of target proteins by directly occupying their DNA binding sites. The extremely divergent amino acid sequences of the DNA mimics make these proteins hard to predict, and although they are likely to be ubiquitous, to date, only a few have been reported and functionally analyzed. Here we used a bioinformatic approach to look for potential DNA mimic proteins among previously reported protein structures. From approximately 14 candidates, we selected the Staphylococcus conserved hypothetical protein SSP0047, and used proteomic and structural approaches to show that it is a novel DNA mimic protein. In Staphylococcus aureus, we found that this protein acts as a uracil-DNA glycosylase inhibitor, and therefore named it S. aureus uracil-DNA glycosylase inhibitor (SAUGI). We also determined and analyzed the complex structure of SAUGI and S. aureus uracil-DNA glycosylase (SAUDG). Subsequent BIAcore studies further showed that SAUGI has a high binding affinity to both S. aureus and human UDG. The two uracil-DNA glycosylase inhibitors (UGI and p56) previously known to science were both found in Bacillus phages, and this is the first report of a bacterial DNA mimic that may regulate SAUDG's functional roles in DNA repair and host defense. Staphylococcus aureus protein SAUGI acts as a uracil-DNA glycosylase inhibitor.,Wang HC, Hsu KC, Yang JM, Wu ML, Ko TP, Lin SR, Wang AH Nucleic Acids Res. 2014 Jan 1;42(2):1354-64. doi: 10.1093/nar/gkt964. Epub 2013, Oct 22. PMID:24150946[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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