1v8m: Difference between revisions
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|PDB= 1v8m |SIZE=350|CAPTION= <scene name='initialview01'>1v8m</scene>, resolution 1.80Å | |PDB= 1v8m |SIZE=350|CAPTION= <scene name='initialview01'>1v8m</scene>, resolution 1.80Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=APR:ADENOSINE-5-DIPHOSPHORIBOSE'>APR</scene> | |LIGAND= <scene name='pdbligand=APR:ADENOSINE-5-DIPHOSPHORIBOSE'>APR</scene>, <scene name='pdbligand=GD:GADOLINIUM+ATOM'>GD</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/ADP-ribose_diphosphatase ADP-ribose diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.13 3.6.1.13] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/ADP-ribose_diphosphatase ADP-ribose diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.13 3.6.1.13] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1v8i|1V8I]], [[1v8l|1V8L]], [[1v8n|1V8N]], [[1v8r|1V8R]], [[1v8s|1V8S]], [[1v8t|1V8T]], [[1v8u|1V8U]], [[1v8v|1V8V]], [[1v8w|1V8W]], [[1v8y|1V8Y]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1v8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v8m OCA], [http://www.ebi.ac.uk/pdbsum/1v8m PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1v8m RCSB]</span> | |||
}} | }} | ||
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[[Category: Yokoyama, S.]] | [[Category: Yokoyama, S.]] | ||
[[Category: Yoshiba, S.]] | [[Category: Yoshiba, S.]] | ||
[[Category: loop-helix-loop]] | [[Category: loop-helix-loop]] | ||
[[Category: mutt family]] | [[Category: mutt family]] | ||
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[[Category: structural genomic]] | [[Category: structural genomic]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:20:59 2008'' |
Revision as of 00:21, 31 March 2008
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, resolution 1.80Å | |||||||
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Ligands: | , | ||||||
Activity: | ADP-ribose diphosphatase, with EC number 3.6.1.13 | ||||||
Related: | 1V8I, 1V8L, 1V8N, 1V8R, 1V8S, 1V8T, 1V8U, 1V8V, 1V8W, 1V8Y
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Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
Coordinates: | save as pdb, mmCIF, xml |
Crystal structure analysis of ADP-ribose pyrophosphatase complexed with ADP-ribose and Gd
OverviewOverview
ADP-ribose pyrophosphatase (ADPRase) catalyzes the divalent metal ion-dependent hydrolysis of ADP-ribose to ribose 5'-phosphate and AMP. This enzyme plays a key role in regulating the intracellular ADP-ribose levels, and prevents nonenzymatic ADP-ribosylation. To elucidate the pyrophosphatase hydrolysis mechanism employed by this enzyme, structural changes occurring on binding of substrate, metal and product were investigated using crystal structures of ADPRase from an extreme thermophile, Thermus thermophilus HB8. Seven structures were determined, including that of the free enzyme, the Zn(2+)-bound enzyme, the binary complex with ADP-ribose, the ternary complexes with ADP-ribose and Zn(2+) or Gd(3+), and the product complexes with AMP and Mg(2+) or with ribose 5'-phosphate and Zn(2+). The structural and functional studies suggested that the ADP-ribose hydrolysis pathway consists of four reaction states: bound with metal (I), metal and substrate (II), metal and substrate in the transition state (III), and products (IV). In reaction state II, Glu-82 and Glu-70 abstract a proton from a water molecule. This water molecule is situated at an ideal position to carry out nucleophilic attack on the adenosyl phosphate, as it is 3.6 A away from the target phosphorus and almost in line with the scissile bond.
About this StructureAbout this Structure
1V8M is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.
ReferenceReference
Structural insights into the Thermus thermophilus ADP-ribose pyrophosphatase mechanism via crystal structures with the bound substrate and metal., Yoshiba S, Ooga T, Nakagawa N, Shibata T, Inoue Y, Yokoyama S, Kuramitsu S, Masui R, J Biol Chem. 2004 Aug 27;279(35):37163-74. Epub 2004 Jun 21. PMID:15210687
Page seeded by OCA on Mon Mar 31 00:20:59 2008
Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Pages with broken file links
- ADP-ribose diphosphatase
- Single protein
- Thermus thermophilus
- Inoue, Y.
- Kuramitsu, S.
- Masui, R.
- Nakagawa, N.
- Ooga, T.
- RSGI, RIKEN Structural Genomics/Proteomics Initiative.
- Shibata, T.
- Yokoyama, S.
- Yoshiba, S.
- Loop-helix-loop
- Mutt family
- Nudix motif
- Riken structural genomics/proteomics initiative
- Rsgi
- Structural genomic