3zgi: Difference between revisions
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==Crystal structure of the KRT10-binding region domain of the pneumococcal serine rich repeat protein PsrP== | ==Crystal structure of the KRT10-binding region domain of the pneumococcal serine rich repeat protein PsrP== | ||
<StructureSection load='3zgi' size='340' side='right' caption='[[3zgi]], [[Resolution|resolution]] 2.25Å' scene=''> | <StructureSection load='3zgi' size='340' side='right' caption='[[3zgi]], [[Resolution|resolution]] 2.25Å' scene=''> | ||
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3zgh|3zgh]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3zgh|3zgh]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zgi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zgi OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zgi RCSB], [http://www.ebi.ac.uk/pdbsum/3zgi PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zgi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zgi OCA], [http://pdbe.org/3zgi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zgi RCSB], [http://www.ebi.ac.uk/pdbsum/3zgi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zgi ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 3zgi" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 11:33, 5 August 2016
Crystal structure of the KRT10-binding region domain of the pneumococcal serine rich repeat protein PsrPCrystal structure of the KRT10-binding region domain of the pneumococcal serine rich repeat protein PsrP
Structural highlights
Publication Abstract from PubMedStreptococcus pneumoniae is a major human pathogen, and a leading cause of disease and death worldwide. Pneumococcal invasive disease is triggered by initial asymptomatic colonization of the human upper respiratory tract. The pneumococcal serine-rich repeat protein (PsrP) is a lung-specific virulence factor whose functional binding region (BR) binds to keratin-10 (KRT10) and promotes pneumococcal biofilm formation through self-oligomerization. We present the crystal structure of the KRT10-binding domain of PsrP (BR187-385) determined to 2.0 A resolution. BR187-385 adopts a novel variant of the DEv-IgG fold, typical for microbial surface components recognizing adhesive matrix molecules adhesins, despite very low sequence identity. An extended beta-sheet on one side of the compressed, two-sided barrel presents a basic groove that possibly binds to the acidic helical rod domain of KRT10. Our study also demonstrates the importance of the other side of the barrel, formed by extensive well-ordered loops and stabilized by short beta-strands, for interaction with KRT10. The basic keratin 10-binding domain of the virulence-associated pneumococcal serine-rich protein PsrP adopts a novel MSCRAMM fold.,Schulte T, Lofling J, Mikaelsson C, Kikhney A, Hentrich K, Diamante A, Ebel C, Normark S, Svergun D, Henriques-Normark B, Achour A Open Biol. 2014 Jan 15;4(1):130090. doi: 10.1098/rsob.130090. PMID:24430336[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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