1sgf: Difference between revisions
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|PDB= 1sgf |SIZE=350|CAPTION= <scene name='initialview01'>1sgf</scene>, resolution 3.15Å | |PDB= 1sgf |SIZE=350|CAPTION= <scene name='initialview01'>1sgf</scene>, resolution 3.15Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene> | |LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Tissue_kallikrein Tissue kallikrein], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.35 3.4.21.35] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Tissue_kallikrein Tissue kallikrein], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.35 3.4.21.35] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sgf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sgf OCA], [http://www.ebi.ac.uk/pdbsum/1sgf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1sgf RCSB]</span> | |||
}} | }} | ||
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[[Category: Mcdonald, N Q.]] | [[Category: Mcdonald, N Q.]] | ||
[[Category: Murray-Rust, J.]] | [[Category: Murray-Rust, J.]] | ||
[[Category: growth factor (beta-ngf)]] | [[Category: growth factor (beta-ngf)]] | ||
[[Category: hydrolase - serine proteinase (gamma-ngf)]] | [[Category: hydrolase - serine proteinase (gamma-ngf)]] | ||
[[Category: inactive serine proteinase (alpha-ngf)]] | [[Category: inactive serine proteinase (alpha-ngf)]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:41:40 2008'' | ||
Revision as of 23:41, 30 March 2008
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| , resolution 3.15Å | |||||||
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| Ligands: | , , | ||||||
| Activity: | Tissue kallikrein, with EC number 3.4.21.35 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF 7S NGF: A COMPLEX OF NERVE GROWTH FACTOR WITH FOUR BINDING PROTEINS (SERINE PROTEINASES)
OverviewOverview
BACKGROUND: Nerve growth factor (NGF) is a neurotrophic factor that promotes the differentiation and survival of certain populations of neurons in the central and peripheral nervous systems. 7S NGF is an alpha 2 beta 2 gamma 2 complex in which the beta-NGF dimer (the active neurotrophin) is associated with two alpha-NGF and two gamma-NGF subunits, which belong to the glandular kallikrein family of serine proteinases. The gamma-NGF subunit is an active serine proteinase capable of processing the precursor form of beta-NGF, whereas alpha-NGF is an inactive serine proteinase. The structure of 7S NGF could be used as a starting point to design inhibitors that prevent NGF binding to its receptors, as a potential treatment of neurodegenerative diseases. RESULTS: The crystal structure of 7S NGF shows that the two gamma-NGF subunits make extensive interactions with each other around the twofold axis of the complex and have the C-terminal residues of the beta-NGF subunits bound within their active sites. The 'activation domain' of each of the alpha-NGF subunits is in an inactive (zymogen-like) conformation and makes extensive interactions with the beta-NGF dimer. The two zinc ions that stabilize the complex are located at the relatively small interfaces between the alpha-NGF and gamma-NGF subunits. CONCLUSIONS: The structure of 7S NGF shows how the twofold axis of the central beta-NGF dimer organizes the symmetry of this multisubunit growth factor complex. The extensive surface of beta-NGF buried within the 7S complex explains the lack of neurotrophic activity observed for 7S NGF. The regions of the beta-NGF dimer that contact the alpha-NGF subunits overlap with those known to engage NGF receptors. Two disulphide-linked loops on alpha-NGF make multiple interactions with beta-NGF and suggest that it might be possible to design peptides that inhibit the binding of beta-NGF to its receptors.
About this StructureAbout this Structure
1SGF is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
ReferenceReference
Structure of mouse 7S NGF: a complex of nerve growth factor with four binding proteins., Bax B, Blundell TL, Murray-Rust J, McDonald NQ, Structure. 1997 Oct 15;5(10):1275-85. PMID:9351801
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