1s68: Difference between revisions
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|PDB= 1s68 |SIZE=350|CAPTION= <scene name='initialview01'>1s68</scene>, resolution 1.90Å | |PDB= 1s68 |SIZE=350|CAPTION= <scene name='initialview01'>1s68</scene>, resolution 1.90Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=AMP:ADENOSINE MONOPHOSPHATE'>AMP</scene> | |LIGAND= <scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= Y10A, 24.1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | |GENE= Y10A, 24.1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 Enterobacteria phage T4]) | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s68 OCA], [http://www.ebi.ac.uk/pdbsum/1s68 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1s68 RCSB]</span> | |||
}} | }} | ||
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==About this Structure== | ==About this Structure== | ||
1S68 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | 1S68 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t4 Enterobacteria phage t4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S68 OCA]. | ||
==Reference== | ==Reference== | ||
Structure and mechanism of RNA ligase., Ho CK, Wang LK, Lima CD, Shuman S, Structure. 2004 Feb;12(2):327-39. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14962393 14962393] | Structure and mechanism of RNA ligase., Ho CK, Wang LK, Lima CD, Shuman S, Structure. 2004 Feb;12(2):327-39. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14962393 14962393] | ||
[[Category: | [[Category: Enterobacteria phage t4]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Ho, C K.]] | [[Category: Ho, C K.]] | ||
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[[Category: Shuman, S.]] | [[Category: Shuman, S.]] | ||
[[Category: Wang, L K.]] | [[Category: Wang, L K.]] | ||
[[Category: ribonucleic acid ligase]] | [[Category: ribonucleic acid ligase]] | ||
[[Category: rna repair]] | [[Category: rna repair]] | ||
[[Category: t4]] | [[Category: t4]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:37:59 2008'' | ||
Revision as of 23:38, 30 March 2008
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| , resolution 1.90Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | Y10A, 24.1 (Enterobacteria phage T4) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure and Mechanism of RNA Ligase
OverviewOverview
T4 RNA ligase 2 (Rnl2) exemplifies an RNA ligase family that includes the RNA editing ligases (RELs) of Trypanosoma and Leishmania. The Rnl2/REL enzymes are defined by essential signature residues and a unique C-terminal domain, which we show is essential for sealing of 3'-OH and 5'-PO4 RNA ends by Rnl2, but not for ligase adenylation or phosphodiester bond formation at a preadenylated AppRNA end. The N-terminal segment Rnl2(1-249) of the 334 aa Rnl2 protein comprises an autonomous adenylyltransferase/AppRNA ligase domain. We report the 1.9 A crystal structure of the ligase domain with AMP bound at the active site, which reveals a shared fold, catalytic mechanism, and evolutionary history for RNA ligases, DNA ligases, and mRNA capping enzymes.
About this StructureAbout this Structure
1S68 is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.
ReferenceReference
Structure and mechanism of RNA ligase., Ho CK, Wang LK, Lima CD, Shuman S, Structure. 2004 Feb;12(2):327-39. PMID:14962393
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