4ilr: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Recognition and Cleavage of a non-structured CRISPR RNA by its Processing Endoribonuclease Cas6== | ==Recognition and Cleavage of a non-structured CRISPR RNA by its Processing Endoribonuclease Cas6== | ||
<StructureSection load='4ilr' size='340' side='right' caption='[[4ilr]], [[Resolution|resolution]] 3.09Å' scene=''> | <StructureSection load='4ilr' size='340' side='right' caption='[[4ilr]], [[Resolution|resolution]] 3.09Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4ilr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[4ilr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulso Sulso]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ILR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ILR FirstGlance]. <br> | ||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3pkm|3pkm]], [[4ill|4ill]], [[4ilm|4ilm]]</td></tr> | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3pkm|3pkm]], [[4ill|4ill]], [[4ilm|4ilm]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cas6b, SSO2004 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cas6b, SSO2004 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 SULSO])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ilr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ilr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ilr RCSB], [http://www.ebi.ac.uk/pdbsum/4ilr PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ilr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ilr OCA], [http://pdbe.org/4ilr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ilr RCSB], [http://www.ebi.ac.uk/pdbsum/4ilr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ilr ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
| Line 15: | Line 16: | ||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4ilr" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Endonuclease|Endonuclease]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Sulso]] | ||
[[Category: Li, H]] | [[Category: Li, H]] | ||
[[Category: Shao, Y]] | [[Category: Shao, Y]] | ||
Revision as of 19:59, 5 August 2016
Recognition and Cleavage of a non-structured CRISPR RNA by its Processing Endoribonuclease Cas6Recognition and Cleavage of a non-structured CRISPR RNA by its Processing Endoribonuclease Cas6
Structural highlights
Publication Abstract from PubMedClustered regularly interspaced short palindromic repeats (CRISPRs) confer adaptive immunity to prokaryotes through a small RNA-mediated mechanism. Specific endoribonucleases are required by all CRISPR-bearing organisms to process CRISPR RNAs into small RNA that serve as guides for defensive effector complexes. The molecular mechanism of how the endoribonucleases process the class of CRISPR RNA containing no predicted secondary structural features remains largely elusive. Here, we report cocrystal structures of a processing endoribonuclease bound with a noncleavable RNA substrate and its product-like fragment derived from a nonpalindramic repeat. The enzyme stabilizes a short RNA stem-loop structure near the cleavage site and cleaves the phosphodiester bond using an active site comprised of arginine and lysine residues. The distinct RNA binding and cleavage mechanisms underline the diversity in CRISPR RNA processing. Recognition and Cleavage of a Nonstructured CRISPR RNA by Its Processing Endoribonuclease Cas6.,Shao Y, Li H Structure. 2013 Feb 27. pii: S0969-2126(13)00017-8. doi:, 10.1016/j.str.2013.01.010. PMID:23454186[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||||