1rwj: Difference between revisions
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|PDB= 1rwj |SIZE=350|CAPTION= <scene name='initialview01'>1rwj</scene>, resolution 1.70Å | |PDB= 1rwj |SIZE=350|CAPTION= <scene name='initialview01'>1rwj</scene>, resolution 1.70Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN IX CONTAINING FE'>HEM</scene> | |LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= ORF03300 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=35554 Geobacter sulfurreducens]) | |GENE= ORF03300 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=35554 Geobacter sulfurreducens]) | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1os6|1os6]], [[1hh5|1hh5]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rwj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rwj OCA], [http://www.ebi.ac.uk/pdbsum/1rwj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rwj RCSB]</span> | |||
}} | }} | ||
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[[Category: Salgueiro, C A.]] | [[Category: Salgueiro, C A.]] | ||
[[Category: Schiffer, M.]] | [[Category: Schiffer, M.]] | ||
[[Category: cytochrome c7]] | [[Category: cytochrome c7]] | ||
[[Category: geobacter metallireducen]] | [[Category: geobacter metallireducen]] | ||
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[[Category: multiheme cytochrome c]] | [[Category: multiheme cytochrome c]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:33:54 2008'' | ||
Revision as of 23:33, 30 March 2008
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| , resolution 1.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | ORF03300 (Geobacter sulfurreducens) | ||||||
| Related: | 1os6, 1hh5
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
c7-type three-heme cytochrome domain
OverviewOverview
The structure of a novel c(7)-type cytochrome domain that has two bishistidine coordinated hemes and one heme with histidine, methionine coordination (where the sixth ligand is a methionine residue) was determined at 1.7 A resolution. This domain is a representative of domains that form three polymers encoded by the Geobacter sulfurreducens genome. Two of these polymers consist of four and one protein of nine c(7)-type domains with a total of 12 and 27 hemes, respectively. Four individual domains (termed A, B, C, and D) from one such multiheme cytochrome c (ORF03300) were cloned and expressed in Escherichia coli. The domain C produced diffraction quality crystals from 2.4 M sodium malonate (pH 7). The structure was solved by MAD method and refined to an R-factor of 19.5% and R-free of 21.8%. Unlike the two c(7) molecules with known structures, one from G. sulfurreducens (PpcA) and one from Desulfuromonas acetoxidans where all three hemes are bishistidine coordinated, this domain contains a heme which is coordinated by a methionine and a histidine residue. As a result, the corresponding heme could have a higher potential than the other two hemes. The apparent midpoint reduction potential, E(app), of domain C is -105 mV, 50 mV higher than that of PpcA.
About this StructureAbout this Structure
1RWJ is a Single protein structure of sequence from Geobacter sulfurreducens. Full crystallographic information is available from OCA.
ReferenceReference
Structure of a novel c7-type three-heme cytochrome domain from a multidomain cytochrome c polymer., Pokkuluri PR, Londer YY, Duke NE, Erickson J, Pessanha M, Salgueiro CA, Schiffer M, Protein Sci. 2004 Jun;13(6):1684-92. Epub 2004 May 7. PMID:15133162
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