1rc7: Difference between revisions
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|PDB= 1rc7 |SIZE=350|CAPTION= <scene name='initialview01'>1rc7</scene>, resolution 2.15Å | |PDB= 1rc7 |SIZE=350|CAPTION= <scene name='initialview01'>1rc7</scene>, resolution 2.15Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene> | |LIGAND= <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3] </span> | ||
|GENE= RNC, AQ_946 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus]) | |GENE= RNC, AQ_946 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus]) | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1i4s|1I4S]], [[1jfz|1JFZ]], [[1o0w|1O0W]], [[1rc5|1RC5]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rc7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rc7 OCA], [http://www.ebi.ac.uk/pdbsum/1rc7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rc7 RCSB]</span> | |||
}} | }} | ||
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[[Category: Gan, J.]] | [[Category: Gan, J.]] | ||
[[Category: Ji, X.]] | [[Category: Ji, X.]] | ||
[[Category: ds-rna]] | [[Category: ds-rna]] | ||
[[Category: endonucleolytic cleavage]] | [[Category: endonucleolytic cleavage]] | ||
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[[Category: rna interference]] | [[Category: rna interference]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:26:10 2008'' | ||
Revision as of 23:26, 30 March 2008
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| , resolution 2.15Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Gene: | RNC, AQ_946 (Aquifex aeolicus) | ||||||
| Activity: | Ribonuclease III, with EC number 3.1.26.3 | ||||||
| Related: | 1I4S, 1JFZ, 1O0W, 1RC5
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of RNase III Mutant E110K from Aquifex Aeolicus complexed with ds-RNA at 2.15 Angstrom Resolution
OverviewOverview
Ribonuclease III (RNase III) represents a family of double-stranded RNA (dsRNA) endonucleases. The simplest bacterial enzyme contains an endonuclease domain (endoND) and a dsRNA binding domain (dsRBD). RNase III can affect RNA structure and gene expression in either of two ways: as a dsRNA-processing enzyme that cleaves dsRNA, or as a dsRNA binding protein that binds but does not cleave dsRNA. We previously determined the endoND structure of Aquifex aeolicus RNase III (Aa-RNase III) and modeled a catalytic complex of full-length Aa-RNase III with dsRNA. Here, we present the crystal structure of Aa-RNase III in complex with dsRNA, revealing a noncatalytic assembly. The major differences between the two functional forms of RNase III.dsRNA are the conformation of the protein and the orientation and location of dsRNA. The flexibility of a 7 residue linker between the endoND and dsRBD enables the transition between these two forms.
About this StructureAbout this Structure
1RC7 is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.
ReferenceReference
Noncatalytic assembly of ribonuclease III with double-stranded RNA., Blaszczyk J, Gan J, Tropea JE, Court DL, Waugh DS, Ji X, Structure. 2004 Mar;12(3):457-66. PMID:15016361
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