1r6m: Difference between revisions
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|PDB= 1r6m |SIZE=350|CAPTION= <scene name='initialview01'>1r6m</scene>, resolution 2.00Å | |PDB= 1r6m |SIZE=350|CAPTION= <scene name='initialview01'>1r6m</scene>, resolution 2.00Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE ION'>PO4</scene> | |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/tRNA_nucleotidyltransferase tRNA nucleotidyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.56 2.7.7.56] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/tRNA_nucleotidyltransferase tRNA nucleotidyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.56 2.7.7.56] </span> | ||
|GENE= RPH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa]) | |GENE= RPH ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa]) | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1r6l|1R6L]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r6m OCA], [http://www.ebi.ac.uk/pdbsum/1r6m PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1r6m RCSB]</span> | |||
}} | }} | ||
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[[Category: Kim, J H.]] | [[Category: Kim, J H.]] | ||
[[Category: Park, E Y.]] | [[Category: Park, E Y.]] | ||
[[Category: beta-alpha-beta-alpha fold]] | [[Category: beta-alpha-beta-alpha fold]] | ||
[[Category: hexamer]] | [[Category: hexamer]] | ||
[[Category: phosphate bound]] | [[Category: phosphate bound]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:23:57 2008'' | ||
Revision as of 23:23, 30 March 2008
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| , resolution 2.00Å | |||||||
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| Ligands: | |||||||
| Gene: | RPH (Pseudomonas aeruginosa) | ||||||
| Activity: | tRNA nucleotidyltransferase, with EC number 2.7.7.56 | ||||||
| Related: | 1R6L
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure Of The tRNA Processing Enzyme Rnase pH From Pseudomonas Aeruginosa In Complex With Phosphate
OverviewOverview
RNase PH is a phosphate-dependent exoribonuclease that catalyzes the removal of nucleotides at the 3' end of the tRNA precursor, leading to the release of nucleoside diphosphate, and generates the CCA end during the maturation process. The 1.9-A crystal structures of the apo and the phosphate-bound forms of RNase PH from Pseudomonas aeruginosa reveal a monomeric RNase PH with an alpha/beta-fold tightly associated into a hexameric ring structure in the form of a trimer of dimers. A five ion pair network, Glu-63-Arg-74-Asp-116-Arg-77-Asp-118 and an ion-pair Glu-26-Arg-69 that are positioned symmetrically in the trimerization interface play critical roles in the formation of a hexameric ring. Single or double mutations of Arg-69, Arg-74, or Arg-77 in these ion pairs leads to the dissociation of the RNase PH hexamer into dimers without perturbing the overall monomeric structure. The dissociated RNase PH dimer completely lost its binding affinity and catalytic activity against a precursor tRNA. Our structural and mutational analyses of RNase PH demonstrate that the hexameric ring formation is a critical feature for the function of members of the RNase PH family.
About this StructureAbout this Structure
1R6M is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.
ReferenceReference
Probing the functional importance of the hexameric ring structure of RNase PH., Choi JM, Park EY, Kim JH, Chang SK, Cho Y, J Biol Chem. 2004 Jan 2;279(1):755-64. Epub 2003 Oct 22. PMID:14573594
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