3j4b: Difference between revisions
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==Structure of T7 gatekeeper protein (gp11)== | ==Structure of T7 gatekeeper protein (gp11)== | ||
<StructureSection load='3j4b' size='340' side='right' caption='[[3j4b]], [[Resolution|resolution]] 12.00Å' scene=''> | <StructureSection load='3j4b' size='340' side='right' caption='[[3j4b]], [[Resolution|resolution]] 12.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3j4b]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[3j4b]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt7 Bpt7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J4B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J4B FirstGlance]. <br> | ||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3j4a|3j4a]]</td></tr> | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3j4a|3j4a]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10760 | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10760 BPT7])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j4b OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j4b RCSB], [http://www.ebi.ac.uk/pdbsum/3j4b PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j4b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j4b OCA], [http://pdbe.org/3j4b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3j4b RCSB], [http://www.ebi.ac.uk/pdbsum/3j4b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3j4b ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 3j4b" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Bpt7]] | ||
[[Category: Arranz, R]] | [[Category: Arranz, R]] | ||
[[Category: Carrascosa, J L]] | [[Category: Carrascosa, J L]] | ||
Revision as of 13:23, 5 August 2016
Structure of T7 gatekeeper protein (gp11)Structure of T7 gatekeeper protein (gp11)
Structural highlights
Publication Abstract from PubMedMost bacterial viruses need aspecialized machinery, called "tail", to inject their genomes inside the bacterial cytoplasm without disrupting the cellular integrity. Bacteriophage T7 is a well-characterized member of the Podoviridae family infecting Escherichia coli (E. coli), and it has a short non-contractile tail that assembles sequentially on the viral head after DNA packaging. The T7 tail is a complex of around 2.7 MDa composed of at least four proteins: the connector (gene product 8, gp8), the tail tubular proteins gp11 and gp12 and the fibres (gp17). Using cryo-electron microscopy (cryo-EM) and single particle image reconstruction techniques, we have determined the precise topology of the tail proteins by comparing the structure of the T7 tail extracted from viruses and a complex formed by recombinant gp8, gp11 and gp12 proteins. Furthermore, the order of assembly of the structural components within the complex was deduced from interaction assays with cloned and purified tail proteins. The existence of common folds among similar tail proteins allowed us to obtain pseudo-atomic threaded models of gp8 (connector) and gp11 (gatekeeper) proteins, which were docked into the corresponding cryo-EM volumes of the tail complex. This pseudo-atomic model of the connector-gatekeeper interaction revealed the existence of a common molecular architecture among viruses belonging to the three tailed bacteriophage families, strongly suggesting that a common molecular mechanism has been favoured during evolution to coordinate the transition between DNA packaging and tail assembly. Structural Characterization Of The Bacteriophage T7 Tail Machinery.,Cuervo A, Pulido-Cid M, Chagoyen M, Arranz R, Gonzalez-Garcia VA, Garcia-Doval C, Caston JR, Valpuesta JM, van Raaij MJ, Martin-Benito J, Carrascosa JL J Biol Chem. 2013 Jul 24. PMID:23884409[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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