2m4f: Difference between revisions
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<StructureSection load='2m4f' size='340' side='right' caption='[[2m4f]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | <StructureSection load='2m4f' size='340' side='right' caption='[[2m4f]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2m4f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[2m4f]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Borbn Borbn]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M4F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2M4F FirstGlance]. <br> | ||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4j38|4j38]]</td></tr> | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4j38|4j38]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BbuN40_O26 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=521007 | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BbuN40_O26 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=521007 BORBN])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2m4f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m4f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2m4f RCSB], [http://www.ebi.ac.uk/pdbsum/2m4f PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2m4f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m4f OCA], [http://pdbe.org/2m4f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2m4f RCSB], [http://www.ebi.ac.uk/pdbsum/2m4f PDBsum]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 2m4f" style="background-color:#fffaf0;"></div> | |||
==See Also== | ==See Also== | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Borbn]] | ||
[[Category: Bhattacharjee, A]] | [[Category: Bhattacharjee, A]] | ||
[[Category: Goldman, A]] | [[Category: Goldman, A]] | ||
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[[Category: Oeemig, J S]] | [[Category: Oeemig, J S]] | ||
[[Category: Bbcrasp-3]] | [[Category: Bbcrasp-3]] | ||
[[Category: Borrelia burgdorferi n40]] | |||
[[Category: Complement]] | [[Category: Complement]] | ||
[[Category: Erp]] | [[Category: Erp]] | ||
Revision as of 23:42, 10 September 2015
Solution Structure of Outer surface protein ESolution Structure of Outer surface protein E
Structural highlights
Publication Abstract from PubMedBorrelia burgdorferi spirochetes that cause Lyme borreliosis survive for a long time in human serum because they successfully evade the complement system, an important arm of innate immunity. The outer surface protein E (OspE) of B. burgdorferi is needed for this because it recruits complement regulator factor H (FH) onto the bacterial surface to evade complement-mediated cell lysis. To understand this process at the molecular level, we used a structural approach. First, we solved the solution structure of OspE by NMR, revealing a fold that has not been seen before in proteins involved in complement regulation. Next, we solved the x-ray structure of the complex between OspE and the FH C-terminal domains 19 and 20 (FH19-20) at 2.83 A resolution. The structure shows that OspE binds FH19-20 in a way similar to, but not identical with, that used by endothelial cells to bind FH via glycosaminoglycans. The observed interaction of OspE with FH19-20 allows the full function of FH in down-regulation of complement activation on the bacteria. This reveals the molecular basis for how B. burgdorferi evades innate immunity and suggests how OspE could be used as a potential vaccine antigen. Structural Basis for Complement Evasion by Lyme Disease Pathogen Borrelia burgdorferi.,Bhattacharjee A, Oeemig JS, Kolodziejczyk R, Meri T, Kajander T, Lehtinen MJ, Iwai H, Jokiranta TS, Goldman A J Biol Chem. 2013 Jun 28;288(26):18685-95. doi: 10.1074/jbc.M113.459040. Epub, 2013 May 8. PMID:23658013[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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