1par: Difference between revisions
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|PDB= 1par |SIZE=350|CAPTION= <scene name='initialview01'>1par</scene>, resolution 2.600Å | |PDB= 1par |SIZE=350|CAPTION= <scene name='initialview01'>1par</scene>, resolution 2.600Å | ||
|SITE= | |SITE= | ||
|LIGAND= | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1par FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1par OCA], [http://www.ebi.ac.uk/pdbsum/1par PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1par RCSB]</span> | |||
}} | }} | ||
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==About this Structure== | ==About this Structure== | ||
1PAR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | 1PAR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PAR OCA]. | ||
==Reference== | ==Reference== | ||
DNA recognition by beta-sheets in the Arc repressor-operator crystal structure., Raumann BE, Rould MA, Pabo CO, Sauer RT, Nature. 1994 Feb 24;367(6465):754-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8107872 8107872] | DNA recognition by beta-sheets in the Arc repressor-operator crystal structure., Raumann BE, Rould MA, Pabo CO, Sauer RT, Nature. 1994 Feb 24;367(6465):754-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8107872 8107872] | ||
[[Category: Enterobacteria phage p22]] | |||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Pabo, C O.]] | [[Category: Pabo, C O.]] | ||
[[Category: Raumann, B E.]] | [[Category: Raumann, B E.]] | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:57:15 2008'' | ||
Revision as of 22:57, 30 March 2008
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| , resolution 2.600Å | |||||||
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| Ligands: | , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
DNA RECOGNITION BY BETA-SHEETS IN THE ARC REPRESSOR-OPERATOR CRYSTAL STRUCTURE
OverviewOverview
Transcription of the ant gene during lytic growth of bacteriophage P22 (ref. 1) is regulated by the cooperative binding of two Arc repressor dimers to a 21-base-pair operator site. Here we report the co-crystal structure of this Arc tetramer-operator complex at 2.6 A resolution. As expected from genetic and structural studies and from the co-crystal structure of the homologous Escherichia coli MetJ repressor, each Arc dimer uses an antiparallel beta-sheet to recognize bases in the major groove. However, the Arc and MetJ complexes differ in several important ways: the beta-sheet-DNA interactions of Arc are far less symmetrical; DNA binding by Arc is accompanied by important conformational changes in the beta-sheet; and Arc uses a different part of its protein surface for dimer-dimer interactions.
About this StructureAbout this Structure
1PAR is a Single protein structure of sequence from Enterobacteria phage p22. Full crystallographic information is available from OCA.
ReferenceReference
DNA recognition by beta-sheets in the Arc repressor-operator crystal structure., Raumann BE, Rould MA, Pabo CO, Sauer RT, Nature. 1994 Feb 24;367(6465):754-7. PMID:8107872
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