4by5: Difference between revisions
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==Crystal structure of Drosophila Frq2== | ==Crystal structure of Drosophila Frq2== | ||
<StructureSection load='4by5' size='340' side='right' caption='[[4by5]], [[Resolution|resolution]] 2.22Å' scene=''> | <StructureSection load='4by5' size='340' side='right' caption='[[4by5]], [[Resolution|resolution]] 2.22Å' scene=''> | ||
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4by4|4by4]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4by4|4by4]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4by5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4by5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4by5 RCSB], [http://www.ebi.ac.uk/pdbsum/4by5 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4by5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4by5 OCA], [http://pdbe.org/4by5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4by5 RCSB], [http://www.ebi.ac.uk/pdbsum/4by5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4by5 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4by5" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 13:27, 5 August 2016
Crystal structure of Drosophila Frq2Crystal structure of Drosophila Frq2
Structural highlights
Publication Abstract from PubMedThe conserved Ca2+-binding protein Frequenin/Neuronal Calcium Sensor 1 (Frq/NCS-1) is involved in pathologies that result from abnormal synapse number and probability of neurotransmitter release per synapse. Both synaptic features are likely co-regulated but the intervening mechanisms remain poorly understood. We show here that Drosophila Ric8a/Synembryn, a receptor-independent activator of G protein complexes, binds Frq2 but not the virtually identical duplicate Frq1. Based on crystallographic data on Frq2 and site directed mutagenesis on Frq1, the differential amino acids R94 and T138 account for this specificity. Human NCS-1 and Ric8a reproduce the binding and maintain the structural requirements at these key positions. Ric8a and Galphas regulate synapse number and neurotransmitter release and both are functionally linked to Frq2. Frq2 negatively regulates Ric8a to control synapse number. However, the regulation of neurotransmitter release by Ric8a is independent from the Frq2 binding. Thus, the antagonistic regulation of these two synaptic properties shares a common pathway, Frq2,Ric8a,Galphas which diverges downstream. These mechanisms expose the Frq2-Ric8a interacting surface as a potential pharmacological target for NCS-1 related diseases and provide key data towards the corresponding drug design. The guanine-exchange factor Ric8a binds the calcium sensor NCS-1 to regulate synapse number and probability of release.,Romero-Pozuelo J, Dason JS, Mansilla A, Banos-Mateos S, Sardina JL, Chaves-Sanjuan A, Jurado-Gomez J, Santana E, Atwood HL, Hernandez-Hernandez A, Sanchez-Barrena MJ, Ferrus A J Cell Sci. 2014 Jul 29. pii: jcs.152603. PMID:25074811[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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