Molecular Playground/OmpG: Difference between revisions
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<Structure load='2GTG' size='500' frame='true' align='right' caption='Outer Membrane Protein G' scene='User:Christina_Chisholm/Sandbox_1/Greenwoselection_ompg/5'> | |||
==Outer Membrane Protein G== | ==Outer Membrane Protein G== | ||
Protein biosensors serve as an analytical device combining a biological component with a physiochemical detector. Through protein engineering we are able to utilize pores to be used as stochastic sensors, for single molecule detection. The applications of biosensors range from fundamental research, clinical diagnosis, and even advances in homeland security. PCR (Polymerase chain reaction) and ELISA (Enzyme- linked immunosorbent assay) are current sensitive detection methods. However, these methods are time consuming and require laborious effort, where results are provided hours or days later. For this reason alternate approaches are mounting in demands that are rapid in detection time, highly sensitive and reliable. | Protein biosensors serve as an analytical device combining a biological component with a physiochemical detector. Through protein engineering we are able to utilize pores to be used as stochastic sensors, for single molecule detection. The applications of biosensors range from fundamental research, clinical diagnosis, and even advances in homeland security. PCR (Polymerase chain reaction) and ELISA (Enzyme- linked immunosorbent assay) are current sensitive detection methods. However, these methods are time consuming and require laborious effort, where results are provided hours or days later. For this reason alternate approaches are mounting in demands that are rapid in detection time, highly sensitive and reliable. | ||
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[3] Damaghi M, Bippes C, et al. (2010) "pH-dependent interactions guide the folding and gate the transmembrane pore of the beta-barrel membrane protein OmpG." J Mol Biol 397(4):878-82. | [3] Damaghi M, Bippes C, et al. (2010) "pH-dependent interactions guide the folding and gate the transmembrane pore of the beta-barrel membrane protein OmpG." J Mol Biol 397(4):878-82. | ||