4heq: Difference between revisions
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==The crystal structure of flavodoxin from Desulfovibrio gigas== | ==The crystal structure of flavodoxin from Desulfovibrio gigas== | ||
<StructureSection load='4heq' size='340' side='right' caption='[[4heq]], [[Resolution|resolution]] 1.30Å' scene=''> | <StructureSection load='4heq' size='340' side='right' caption='[[4heq]], [[Resolution|resolution]] 1.30Å' scene=''> | ||
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<table><tr><td colspan='2'>[[4heq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_gigas Desulfovibrio gigas]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HEQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HEQ FirstGlance]. <br> | <table><tr><td colspan='2'>[[4heq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_gigas Desulfovibrio gigas]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HEQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HEQ FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4heq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4heq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4heq RCSB], [http://www.ebi.ac.uk/pdbsum/4heq PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4heq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4heq OCA], [http://pdbe.org/4heq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4heq RCSB], [http://www.ebi.ac.uk/pdbsum/4heq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4heq ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4heq" style="background-color:#fffaf0;"></div> | |||
==See Also== | ==See Also== | ||
Revision as of 22:54, 15 December 2016
The crystal structure of flavodoxin from Desulfovibrio gigasThe crystal structure of flavodoxin from Desulfovibrio gigas
Structural highlights
Publication Abstract from PubMedFlavodoxins, which exist widely in microorganisms, have been found in various pathways with multiple physiological functions. The flavodoxin (Fld) containing the cofactor flavin mononucleotide (FMN) from sulfur-reducing bacteria Desulfovibrio gigas (D. gigas) is a short-chain enzyme that comprises 146 residues with a molecular mass of 15 kDa and plays important roles in the electron-transfer chain. To investigate its structure, we purified this Fld directly from anaerobically grown D. gigas cells. The crystal structure of Fld, determined at resolution 1.3 A, is a dimer with two FMN packing in an orientation head to head at a distance of 17 A, which generates a long and connected negatively charged region. Two loops, Thr59-Asp63 and Asp95-Tyr100, are located in the negatively charged region and between two FMN, and are structurally dynamic. An analysis of each monomer shows that the structure of Fld is in a semiquinone state; the positions of FMN and the surrounding residues in the active site deviate. The crystal structure of Fld from D. gigas agrees with a dimeric form in the solution state. The dimerization area, dynamic characteristics and structure variations between monomers enable us to identify a possible binding area for its functional partners. Crystal Structure of Dimeric Flavodoxin from Desulfovibrio gigas Suggests a Potential Binding Region for the Electron-Transferring Partner.,Hsieh YC, Chia TS, Fun HK, Chen CJ Int J Mol Sci. 2013 Jan 15;14(1):1667-83. doi: 10.3390/ijms14011667. PMID:23322018[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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