4dyc: Difference between revisions
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==Crystal Structure of the terminase small subunit gp1 with D19R mutation of the bacterial virus sf6== | ==Crystal Structure of the terminase small subunit gp1 with D19R mutation of the bacterial virus sf6== | ||
<StructureSection load='4dyc' size='340' side='right' caption='[[4dyc]], [[Resolution|resolution]] 1.80Å' scene=''> | <StructureSection load='4dyc' size='340' side='right' caption='[[4dyc]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4dyc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[4dyc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bphk6 Bphk6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DYC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DYC FirstGlance]. <br> | ||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3hef|3hef]], [[4dzp|4dzp]], [[4dyr|4dyr]], [[4dzj|4dzj]], [[4dyq|4dyq]]</td></tr> | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3hef|3hef]], [[4dzp|4dzp]], [[4dyr|4dyr]], [[4dzj|4dzj]], [[4dyq|4dyq]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gp1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=155148 | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gp1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=155148 BPHK6])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dyc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dyc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4dyc RCSB], [http://www.ebi.ac.uk/pdbsum/4dyc PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dyc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dyc OCA], [http://pdbe.org/4dyc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4dyc RCSB], [http://www.ebi.ac.uk/pdbsum/4dyc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4dyc ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4dyc" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Bphk6]] | ||
[[Category: H., Zhao]] | [[Category: H., Zhao]] | ||
[[Category: L., Tang]] | [[Category: L., Tang]] | ||
Revision as of 05:44, 11 December 2016
Crystal Structure of the terminase small subunit gp1 with D19R mutation of the bacterial virus sf6Crystal Structure of the terminase small subunit gp1 with D19R mutation of the bacterial virus sf6
Structural highlights
Publication Abstract from PubMedIn many DNA viruses, genome packaging is initiated by the small subunit of the packaging terminase, which specifically binds to the packaging signal on viral DNA and directs assembly of the terminase holoenzyme. We have experimentally mapped the DNA-interacting region on Shigella virus Sf6 terminase small subunit gp1, which occupies extended surface areas encircling the gp1 octamer, indicating that DNA wraps around gp1 through extensive contacts. High-resolution structures reveal large-scale motions of the gp1 DNA-binding domain mediated by the curved helix formed by residues 54-81 and an intermolecular salt bridge formed by residues Arg67 and Glu73, indicating remarkable structural plasticity underlying multivalent, pleomorphic gp1:DNA interactions. These results provide spatial restraints for protein:DNA interactions, which enable construction of a three-dimensional pseudo-atomic model for a DNA-packaging initiation complex assembled from the terminase small subunit and the packaging region on viral DNA. Our results suggest that gp1 functions as a DNA-spooling device, which may transform DNA into a specific architecture appropriate for interaction with and cleavage by the terminase large subunit prior to DNA translocation into viral procapsid. This may represent a common mechanism for the initiation step of DNA packaging in tailed double-stranded DNA bacterial viruses. Structural and Functional Studies of the Phage Sf6 Terminase Small Subunit Reveal a DNA-Spooling Device Facilitated by Structural Plasticity.,Zhao H, Kamau YN, Christensen TE, Tang L J Mol Biol. 2012 Oct 26;423(3):413-26. doi: 10.1016/j.jmb.2012.07.016. Epub 2012 , Jul 31. PMID:22858866[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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