1nt9: Difference between revisions
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|SITE= | |SITE= | ||
|LIGAND= | |LIGAND= | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1i3q|1I3Q]], [[1i50|1I50]], [[1i6h|1I6H]], [[1g03|1G03]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nt9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nt9 OCA], [http://www.ebi.ac.uk/pdbsum/1nt9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1nt9 RCSB]</span> | |||
}} | }} | ||
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[[Category: Cramer, P.]] | [[Category: Cramer, P.]] | ||
[[Category: Kettenberger, H.]] | [[Category: Kettenberger, H.]] | ||
[[Category: dna-dependent rna polymerase | [[Category: cellular rna polymerase]] | ||
[[Category: dna-dependent rna polymerase]] | |||
[[Category: gene expression]] | |||
[[Category: messenger rna synthesis]] | |||
[[Category: mrna]] | |||
[[Category: multisubunit complex]] | |||
[[Category: transcription]] | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:35:09 2008'' | ||
Revision as of 22:35, 30 March 2008
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| , resolution 4.20Å | |||||||
|---|---|---|---|---|---|---|---|
| Activity: | DNA-directed RNA polymerase, with EC number 2.7.7.6 | ||||||
| Related: | 1I3Q, 1I50, 1I6H, 1G03
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Complete 12-subunit RNA polymerase II
OverviewOverview
RNA polymerase (Pol) II consists of a 10-polypeptide catalytic core and the two-subunit Rpb4/7 complex that is required for transcription initiation. Previous structures of the Pol II core revealed a "clamp," which binds the DNA template strand via three "switch regions," and a flexible "linker" to the C-terminal repeat domain (CTD). Here we derived a model of the complete Pol II by fitting structures of the core and Rpb4/7 to a 4.2-A crystallographic electron density map. Rpb4/7 protrudes from the polymerase "upstream face," on which initiation factors assemble for promoter DNA loading. Rpb7 forms a wedge between the clamp and the linker, restricting the clamp to a closed position. The wedge allosterically prevents entry of the promoter DNA duplex into the active center cleft and induces in two switch regions a conformation poised for template-strand binding. Interaction of Rpb4/7 with the linker explains Rpb4-mediated recruitment of the CTD phosphatase to the CTD during Pol II recycling. The core-Rpb7 interaction and some functions of Rpb4/7 are apparently conserved in all eukaryotic and archaeal RNA polymerases but not in the bacterial enzyme.
About this StructureAbout this Structure
1NT9 is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
ReferenceReference
Architecture of initiation-competent 12-subunit RNA polymerase II., Armache KJ, Kettenberger H, Cramer P, Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):6964-8. Epub 2003 May 13. PMID:12746495
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