3dw5: Difference between revisions
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==Crystal Structure of the Sarcin/Ricin Domain from E. COLI 23S rRNA, U2656-OCH3 modified== | ==Crystal Structure of the Sarcin/Ricin Domain from E. COLI 23S rRNA, U2656-OCH3 modified== | ||
<StructureSection load='3dw5' size='340' side='right' caption='[[3dw5]], [[Resolution|resolution]] 0.96Å' scene=''> | <StructureSection load='3dw5' size='340' side='right' caption='[[3dw5]], [[Resolution|resolution]] 0.96Å' scene=''> | ||
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</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene></td></tr> | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OMU:O2-METHYLURIDINE+5-MONOPHOSPHATE'>OMU</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1q9a|1q9a]], [[3dvz|3dvz]], [[3dw4|3dw4]], [[3dw6|3dw6]], [[3dw7|3dw7]], [[483d|483d]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1q9a|1q9a]], [[3dvz|3dvz]], [[3dw4|3dw4]], [[3dw6|3dw6]], [[3dw7|3dw7]], [[483d|483d]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dw5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dw5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3dw5 RCSB], [http://www.ebi.ac.uk/pdbsum/3dw5 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3dw5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3dw5 OCA], [http://pdbe.org/3dw5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3dw5 RCSB], [http://www.ebi.ac.uk/pdbsum/3dw5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3dw5 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 3dw5" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 11:20, 11 August 2016
Crystal Structure of the Sarcin/Ricin Domain from E. COLI 23S rRNA, U2656-OCH3 modifiedCrystal Structure of the Sarcin/Ricin Domain from E. COLI 23S rRNA, U2656-OCH3 modified
Structural highlights
Publication Abstract from PubMedSite-specific 2'-methylseleno RNA labeling is a promising tool for tackling the phase problem in RNA crystallography. We have developed an efficient strategy for crystallization and structure determination of RNA and RNA/protein complexes based on preliminary crystallization screening of 2'-OCH(3)-modified RNA sequences, prior to the replacement of 2'-OCH(3) groups with their 2'-SeCH(3) counterparts. The method exploits the similar crystallization properties of 2'-OCH(3)- and 2'-SeCH(3)-modified RNAs and has been successfully validated for two test cases. In addition, our data show that 2'-SeCH(3)-modified RNA have an increased resistance to X-ray radiolysis in comparison with commonly used 5-halogen-modified RNA, which permits collection of experimental electron density maps of remarkable quality. A fast selenium derivatization strategy for crystallization and phasing of RNA structures.,Olieric V, Rieder U, Lang K, Serganov A, Schulze-Briese C, Micura R, Dumas P, Ennifar E RNA. 2009 Apr;15(4):707-15. Epub 2009 Feb 18. PMID:19228585[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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