1ipa: Difference between revisions
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ipa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ipa OCA], [http://www.ebi.ac.uk/pdbsum/1ipa PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ipa RCSB]</span> | |||
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[[Category: structural genomic]] | [[Category: structural genomic]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:22:48 2008'' | ||
Revision as of 21:22, 30 March 2008
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF RNA 2'-O RIBOSE METHYLTRANSFERASE
OverviewOverview
Knots in polypeptide chains have been found in very few proteins. Only two proteins are considered to have a shallow 'trefoil' knot, which tucks a few residues at one end of the chain through a loop exposed on the protein surface. Recently, another protein was found by a mathematical algorithm to have a deep 'figure-of-eight' knot which had not been visually identified. In the present study, the crystal structure of a hypothetical RNA 2'-O-ribose methyltransferase from Thermus thermophilus (RrmA) was determined at 2.4 A resolution and a deep trefoil knot was found for the first time. The present knot is formed by the threading of a 44-residue polypeptide chain through a 41-residue loop and is better defined than the previously reported knots. Two of the three catalytic residues conserved in the 2'-O-ribose methyltransferase family are located in the knotting loop and in the knotted carboxy-terminal chain, which is the first observation that the enzyme active site is constructed right on the knot. On the other hand, the amino-terminal domain exhibits a geometrical similarity to the ribosomal proteins which recognize an internal loop of RNA.
About this StructureAbout this Structure
1IPA is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.
ReferenceReference
An enzyme with a deep trefoil knot for the active-site architecture., Nureki O, Shirouzu M, Hashimoto K, Ishitani R, Terada T, Tamakoshi M, Oshima T, Chijimatsu M, Takio K, Vassylyev DG, Shibata T, Inoue Y, Kuramitsu S, Yokoyama S, Acta Crystallogr D Biol Crystallogr. 2002 Jul;58(Pt 7):1129-37. Epub 2002, Jun 20. PMID:12077432
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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Pages with broken file links
- Single protein
- Thermus thermophilus
- Chijimatsu, M.
- Hashimoto, K.
- Inoue, Y.
- Ishitani, R.
- Kuramitsu, S.
- Nureki, O.
- Oshima, T.
- RSGI, RIKEN Structural Genomics/Proteomics Initiative.
- Shibata, T.
- Shirouzu, M.
- Takio, K.
- Tamakoshi, M.
- Terada, T.
- Vassylyev, D G.
- Yokoyama, S.
- Deep trefoil knot
- El30-like fold
- Riken structural genomics/proteomics initiative
- Rossmann fold
- Rsgi
- Structural genomic