3j7z: Difference between revisions

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-'''Unreleased structure'''
+==Structure of the E. coli 50S subunit with ErmCL nascent chain==
+<StructureSection load='3j7z' size='340' side='right' caption='[[3j7z]], [[Resolution|resolution]] 3.90&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3j7z]] is a 34 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12] and [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J7Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J7Z FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ERY:ERYTHROMYCIN+A'>ERY</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j7z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j7z RCSB], [http://www.ebi.ac.uk/pdbsum/3j7z PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+During protein synthesis, nascent polypeptide chains within the ribosomal tunnel can act in cis to induce ribosome stalling and regulate expression of downstream genes. The Staphylococcus aureus ErmCL leader peptide induces stalling in the presence of clinically important macrolide antibiotics, such as erythromycin, leading to the induction of the downstream macrolide resistance methyltransferase ErmC. Here, we present a cryo-electron microscopy (EM) structure of the erythromycin-dependent ErmCL-stalled ribosome at 3.9 A resolution. The structure reveals how the ErmCL nascent chain directly senses the presence of the tunnel-bound drug and thereby induces allosteric conformational rearrangements at the peptidyltransferase center (PTC) of the ribosome. ErmCL-induced perturbations of the PTC prevent stable binding and accommodation of the aminoacyl-tRNA at the A-site, leading to inhibition of peptide bond formation and translation arrest.
-The entry 3j7z is ON HOLD  until Paper Publication
+Drug Sensing by the Ribosome Induces Translational Arrest via Active Site Perturbation.,Arenz S, Meydan S, Starosta AL, Berninghausen O, Beckmann R, Vazquez-Laslop N, Wilson DN Mol Cell. 2014 Oct 1. pii: S1097-2765(14)00746-1. doi:, 10.1016/j.molcel.2014.09.014. PMID:25306253<ref>PMID:25306253</ref>
-Authors: Arenz, S., Meydan, S., Starosta, A.L., Berninghausen, O., Beckmann, R., Vazquez-Laslop, N., Wilson, D.N.
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-Description: Structure of the E. coli 50S subunit with ErmCL nascent chain
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Escherichia coli k-12]]
+[[Category: Staphylococcus aureus]]
+[[Category: Arenz, S.]]
+[[Category: Beckmann, R.]]
+[[Category: Berninghausen, O.]]
+[[Category: Meydan, S.]]
+[[Category: Starosta, A L.]]
+[[Category: Vazquez-Laslop, N.]]
+[[Category: Wilson, D N.]]
+[[Category: Erythromycin]]
+[[Category: Ribosome-antibiotic complex]]
+[[Category: Stalling]]