2k2m: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2k2m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K2M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2K2M FirstGlance]. <br> | <table><tr><td colspan='2'>[[2k2m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K2M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2K2M FirstGlance]. <br> | ||
</td></tr><tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EPS8L1, DRC3, EPS8R1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr> | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EPS8L1, DRC3, EPS8R1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr> | ||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k2m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k2m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2k2m RCSB], [http://www.ebi.ac.uk/pdbsum/2k2m PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k2m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k2m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2k2m RCSB], [http://www.ebi.ac.uk/pdbsum/2k2m PDBsum]</span></td></tr> | ||
<table> | </table> | ||
== Function == | |||
[[http://www.uniprot.org/uniprot/ES8L1_HUMAN ES8L1_HUMAN]] Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton.<ref>PMID:14565974</ref> | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Aitio, O | [[Category: Aitio, O]] | ||
[[Category: Hellman, M | [[Category: Hellman, M]] | ||
[[Category: Kesti, T | [[Category: Kesti, T]] | ||
[[Category: Kleino, I | [[Category: Kleino, I]] | ||
[[Category: Permi, P | [[Category: Permi, P]] | ||
[[Category: Saksela, K | [[Category: Saksela, K]] | ||
[[Category: Samuilova, O | [[Category: Samuilova, O]] | ||
[[Category: Tossavainen, H | [[Category: Tossavainen, H]] | ||
[[Category: Sh3 domain]] | [[Category: Sh3 domain]] | ||
[[Category: Signaling protein]] | [[Category: Signaling protein]] | ||
Revision as of 19:25, 25 December 2014
Structural Basis of PxxDY Motif Recognition in SH3 BindingStructural Basis of PxxDY Motif Recognition in SH3 Binding
Structural highlights
Function[ES8L1_HUMAN] Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton.[1] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedWe have determined the solution structure of epidermal growth factor receptor pathway substrate 8 (Eps8) L1 Src homology 3 (SH3) domain in complex with the PPVPNPDYEPIR peptide from the CD3epsilon cytoplasmic tail. Our structure reveals the distinct structural features that account for the unusual specificity of the Eps8 family SH3 domains for ligands containing a PxxDY motif instead of canonical PxxP ligands. The CD3epsilon peptide binds Eps8L1 SH3 in a class II orientation, but neither adopts a polyproline II helical conformation nor engages the first proline-binding pocket of the SH3 ligand binding interface. Ile531 of Eps8L1 SH3, instead of Tyr or Phe residues typically found in this position in SH3 domains, renders this hydrophobic pocket smaller and nonoptimal for binding to conventional PxxP peptides. A positively charged arginine at position 512 in the n-Src loop of Eps8L1 SH3 plays a key role in PxxDY motif recognition by forming a salt bridge to D7 of the CD3epsilon peptide. In addition, our structural model suggests a hydrogen bond between the hydroxyl group of the aromatic ring of Y8 and the carboxyl group of E496, thus explaining the critical role of the PxxDY motif tyrosine residue in binding to Eps8 family SH3. These finding have direct implications also for understanding the atypical binding specificity of the amino-terminal SH3 of the Nck family proteins. Structural basis of PxxDY motif recognition in SH3 binding.,Aitio O, Hellman M, Kesti T, Kleino I, Samuilova O, Paakkonen K, Tossavainen H, Saksela K, Permi P J Mol Biol. 2008 Sep 26;382(1):167-78. Epub 2008 Jul 11. PMID:18644376[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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