1hxh: Difference between revisions
No edit summary |
No edit summary |
||
| Line 5: | Line 5: | ||
|SITE= | |SITE= | ||
|LIGAND= | |LIGAND= | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/3(or_17)-beta-hydroxysteroid_dehydrogenase 3(or 17)-beta-hydroxysteroid dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.51 1.1.1.51] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/3(or_17)-beta-hydroxysteroid_dehydrogenase 3(or 17)-beta-hydroxysteroid dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.51 1.1.1.51] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[2hsd|2hsd]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1hxh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hxh OCA], [http://www.ebi.ac.uk/pdbsum/1hxh PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1hxh RCSB]</span> | |||
}} | }} | ||
| Line 35: | Line 38: | ||
[[Category: short-chain dehydrogenase]] | [[Category: short-chain dehydrogenase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:11:42 2008'' | ||
Revision as of 21:11, 30 March 2008
| |||||||
| , resolution 1.22Å | |||||||
|---|---|---|---|---|---|---|---|
| Activity: | 3(or 17)-beta-hydroxysteroid dehydrogenase, with EC number 1.1.1.51 | ||||||
| Related: | 2hsd
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
COMAMONAS TESTOSTERONI 3BETA/17BETA HYDROXYSTEROID DEHYDROGENASE
OverviewOverview
The enzyme 3beta/17beta-hydroxysteroid dehydrogenase (3beta/17beta-HSD) is a steroid-inducible component of the Gram-negative bacterium Comamonas testosteroni. It catalyzes the reversible reduction/dehydrogenation of the oxo/beta-hydroxy groups at positions 3 and 17 of steroid compounds, including hormones and isobile acids. Crystallographic analysis at 1.2 A resolution reveals the enzyme to have nearly identical subunits that form a tetramer with 222 symmetry. This is one of the largest oligomeric structures refined at this resolution. The subunit consists of a monomer with a single-domain structure built around a seven-stranded beta-sheet flanked by six alpha-helices. The active site contains a Ser-Tyr-Lys triad, typical for short-chain dehydrogenases/reductases (SDR). Despite their highly diverse substrate specificities, SDR members show a close to identical folding pattern architectures and a common catalytic mechanism. In contrast to other SDR apostructures determined, the substrate binding loop is well-defined. Analysis of structure-activity relationships of catalytic cleft residues, docking analysis of substrates and inhibitors, and accessible surface analysis explains how 3beta/17beta-HSD accommodates steroid substrates of different conformations.
About this StructureAbout this Structure
1HXH is a Single protein structure of sequence from Comamonas testosteroni. Full crystallographic information is available from OCA.
ReferenceReference
Structure of bacterial 3beta/17beta-hydroxysteroid dehydrogenase at 1.2 A resolution: a model for multiple steroid recognition., Benach J, Filling C, Oppermann UC, Roversi P, Bricogne G, Berndt KD, Jornvall H, Ladenstein R, Biochemistry. 2002 Dec 17;41(50):14659-68. PMID:12475215
Page seeded by OCA on Sun Mar 30 21:11:42 2008