1h8h: Difference between revisions

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Revision as of 13:03, 23 March 2008

File:1h8h.jpg

, resolution 2.9Å

Sites:

, , , , , , , , , , , and

Ligands:

, , , and

Activity:

Transferred entry: 3.6.3.14, with EC number 3.6.1.34

Coordinates:

save as pdb, mmCIF, xml

BOVINE MITOCHONDRIAL F1-ATPASE CRYSTALLISED IN THE PRESENCE OF 5MM AMPPNP

OverviewOverview

Analysis of tryptophan mutants of F(1)-ATPase from Escherichia coli [Lobau et al. (1997) FEBS Lett. 404, 15-18] suggested that nucleotide concentrations used to grow crystals for the determination of the structure of bovine F(1)-ATPase [Abrahams et al. (1994) Nature 370, 621-628] would be sufficient to occupy only two catalytic sites, and that higher concentrations of nucleotide would result in all three sites being occupied. We have determined the structure of bovine F(1)-ATPase at 2.9 A resolution with crystals grown in the presence of 5 mM AMPPNP and 5 microM ADP. Similar to previous structures of bovine F(1)-ATPase determined with crystals grown in the presence of lower nucleotide concentrations, only two beta-subunits have bound nucleotide and the third subunit remains empty.

About this StructureAbout this Structure

1H8H is a Protein complex structure of sequences from Bos taurus. Full crystallographic information is available from OCA.

ReferenceReference

The structure and nucleotide occupancy of bovine mitochondrial F(1)-ATPase are not influenced by crystallisation at high concentrations of nucleotide., Menz RI, Leslie AG, Walker JE, FEBS Lett. 2001 Apr 6;494(1-2):11-4. PMID:11297725

Page seeded by OCA on Sun Mar 23 12:03:24 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 4: / Line 4: @@
 |PDB= 1h8h |SIZE=350|CAPTION= <scene name='initialview01'>1h8h</scene>, resolution 2.9&Aring;
 |SITE= <scene name='pdbsite=AC1:Atp+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Mg+Binding+Site+For+Chain+A'>AC2</scene>, <scene name='pdbsite=AC3:Atp+Binding+Site+For+Chain+B'>AC3</scene>, <scene name='pdbsite=AC4:Mg+Binding+Site+For+Chain+B'>AC4</scene>, <scene name='pdbsite=AC5:Atp+Binding+Site+For+Chain+C'>AC5</scene>, <scene name='pdbsite=AC6:Mg+Binding+Site+For+Chain+C'>AC6</scene>, <scene name='pdbsite=AC7:Adp+Binding+Site+For+Chain+D'>AC7</scene>, <scene name='pdbsite=AC8:Mg+Binding+Site+For+Chain+D'>AC8</scene>, <scene name='pdbsite=AC9:Po4+Binding+Site+For+Chain+E'>AC9</scene>, <scene name='pdbsite=BC1:Atp+Binding+Site+For+Chain+F'>BC1</scene>, <scene name='pdbsite=BC2:Mg+Binding+Site+For+Chain+F'>BC2</scene>, <scene name='pdbsite=CAT:The+Carboxylate+Group+Of+Glutamic+Aci+Residue+Is+Believe+...'>CAT</scene> and <scene name='pdbsite=PLP:LYS+Within+The+P-Loop+(Phosphate+Binding)+Motif,+Gxxxxgkt/S'>PLP</scene>
-|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ATP:ADENOSINE-5'-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
+|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ATP:ADENOSINE-5&#39;-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=ADP:ADENOSINE-5&#39;-DIPHOSPHATE'>ADP</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
 |ACTIVITY= [http://en.wikipedia.org/wiki/Transferred_entry:_3.6.3.14 Transferred entry: 3.6.3.14], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.34 3.6.1.34]
 |GENE=
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 [[Category: f1fo atp synthase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:33:44 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 12:03:24 2008''