2kqr: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2kqr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Brugia_malayi Brugia malayi]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KQR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KQR FirstGlance]. <br> | <table><tr><td colspan='2'>[[2kqr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Brugia_malayi Brugia malayi]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KQR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KQR FirstGlance]. <br> | ||
</td></tr><tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">asparaginyl-tRNA synthetase ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6279 Brugia malayi])</td></tr> | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">asparaginyl-tRNA synthetase ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6279 Brugia malayi])</td></tr> | ||
<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Asparagine--tRNA_ligase Asparagine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.22 6.1.1.22] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Asparagine--tRNA_ligase Asparagine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.22 6.1.1.22] </span></td></tr> | ||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kqr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kqr RCSB], [http://www.ebi.ac.uk/pdbsum/2kqr PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kqr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kqr RCSB], [http://www.ebi.ac.uk/pdbsum/2kqr PDBsum]</span></td></tr> | ||
<table> | </table> | ||
== Function == | |||
[[http://www.uniprot.org/uniprot/SYNC_BRUMA SYNC_BRUMA]] Potentially protective antigen in lymphatic filariasis. | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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[[Category: Asparagine--tRNA ligase]] | [[Category: Asparagine--tRNA ligase]] | ||
[[Category: Brugia malayi]] | [[Category: Brugia malayi]] | ||
[[Category: | [[Category: Structural genomic]] | ||
[[Category: Kron, M A | [[Category: Kron, M A]] | ||
[[Category: Peterson, F C | [[Category: Peterson, F C]] | ||
[[Category: Volkman, B F | [[Category: Volkman, B F]] | ||
[[Category: Aminoacyl-trna synthetase]] | [[Category: Aminoacyl-trna synthetase]] | ||
[[Category: Atp-binding]] | [[Category: Atp-binding]] | ||
[[Category: Cesg]] | [[Category: Cesg]] | ||
[[Category: Ligase]] | [[Category: Ligase]] | ||
[[Category: Nucleotide-binding]] | [[Category: Nucleotide-binding]] | ||
[[Category: Protein biosynthesis]] | [[Category: Protein biosynthesis]] | ||
[[Category: Protein structure initiative | [[Category: PSI, Protein structure initiative]] | ||
Revision as of 01:39, 25 December 2014
Solution structure of the N-terminal domain (residues 1-111) of Brugia malayi asparaginyl-tRNA synthetaseSolution structure of the N-terminal domain (residues 1-111) of Brugia malayi asparaginyl-tRNA synthetase
Structural highlights
Function[SYNC_BRUMA] Potentially protective antigen in lymphatic filariasis. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedAminoacyl-tRNA synthetases are validated molecular targets for anti-infective drug discovery because of their essentiality in protein synthesis. Thanks to genome sequencing, it is now possible to systematically study aminoacyl-tRNA synthetases from human eukaryotic parasites as putative targets for novel drug discovery. As part of a program targeting class IIb asparaginyl-tRNA synthetases (AsnRS) from the parasitic nematode Brugia malayi for anti-filarial drugs, we report the complete structure of a eukaryotic AsnRS. Metazoan and fungal AsnRS differ from their bacterial homologues by the addition of a conserved N-terminal extension of about 110 residues whose structure we have determined by solution NMR for the B. malayi enzyme. In addition, we solved by X-ray crystallography a series of structures of the catalytically active N-terminally truncated enzyme (residues 112-548), allowing the structural basis for the mechanism of asparagine activation to be elucidated. The N-terminal domain contains a structured region with a novel fold featuring a lysine-rich helix that is shown by NMR to interact with tRNA. This is connected by an unstructured tether to the remainder of the enzyme, which is highly similar to the known structure of bacterial AsnRS. These data enable a model of the complete AsnRS-tRNA complex to be constructed. A Hybrid Structural Model of the Complete Brugia malayi Cytoplasmic Asparaginyl-tRNA Synthetase.,Crepin T, Peterson F, Haertlein M, Jensen D, Wang C, Cusack S, Kron M J Mol Biol. 2010 Dec 4. PMID:21134380[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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