2izx: Difference between revisions
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==Overview== | ==Overview== | ||
Localization of cyclic AMP (cAMP)-dependent protein kinase (PKA) by A, kinase-anchoring proteins (AKAPs) restricts the action of this broad, specificity kinase. The high-resolution crystal structures of the docking, and dimerization (D/D) domain of the RIIalpha regulatory subunit of PKA, both in the apo state and in complex with the high-affinity anchoring, peptide AKAP-IS explain the molecular basis for AKAP-regulatory subunit, recognition. AKAP-IS folds into an amphipathic alpha helix that engages an, essentially preformed shallow groove on the surface of the RII dimer D/D, domains. Conserved AKAP aliphatic residues dominate interactions to RII at, the predominantly hydrophobic interface, whereas polar residues are, important in conferring R subunit isoform specificity. Using a peptide, . | Localization of cyclic AMP (cAMP)-dependent protein kinase (PKA) by A, kinase-anchoring proteins (AKAPs) restricts the action of this broad, specificity kinase. The high-resolution crystal structures of the docking, and dimerization (D/D) domain of the RIIalpha regulatory subunit of PKA, both in the apo state and in complex with the high-affinity anchoring, peptide AKAP-IS explain the molecular basis for AKAP-regulatory subunit, recognition. AKAP-IS folds into an amphipathic alpha helix that engages an, essentially preformed shallow groove on the surface of the RII dimer D/D, domains. Conserved AKAP aliphatic residues dominate interactions to RII at, the predominantly hydrophobic interface, whereas polar residues are, important in conferring R subunit isoform specificity. Using a peptide, screening approach, we have developed SuperAKAP-IS, a peptide that is, 10,000-fold more selective for the RII isoform relative to RI and can be, used to assess the impact of PKA isoform-selective anchoring on, cAMP-responsive events inside cells. | ||
==About this Structure== | ==About this Structure== | ||
2IZX is a | 2IZX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with DTD as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/cAMP-dependent_protein_kinase cAMP-dependent protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.11 2.7.11.11] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IZX OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: pka]] | [[Category: pka]] | ||
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