1l2h: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1l2h]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L2H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1L2H FirstGlance]. <br> | <table><tr><td colspan='2'>[[1l2h]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L2H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1L2H FirstGlance]. <br> | ||
</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[9ilb|9ilb]]</td></tr> | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[9ilb|9ilb]]</td></tr> | ||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1l2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l2h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1l2h RCSB], [http://www.ebi.ac.uk/pdbsum/1l2h PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1l2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l2h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1l2h RCSB], [http://www.ebi.ac.uk/pdbsum/1l2h PDBsum]</span></td></tr> | ||
<table> | </table> | ||
== Function == | |||
[[http://www.uniprot.org/uniprot/IL1B_HUMAN IL1B_HUMAN]] Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.<ref>PMID:3920526</ref> | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Heidary, D K | [[Category: Heidary, D K]] | ||
[[Category: Jennings, P A | [[Category: Jennings, P A]] | ||
[[Category: Kelker, M S | [[Category: Kelker, M S]] | ||
[[Category: Oseroff, V | [[Category: Oseroff, V]] | ||
[[Category: Rudolph, M G | [[Category: Rudolph, M G]] | ||
[[Category: Schneider, T R | [[Category: Schneider, T R]] | ||
[[Category: Wilson, I A | [[Category: Wilson, I A]] | ||
[[Category: Yeates, T O | [[Category: Yeates, T O]] | ||
[[Category: Beta-trefoil]] | [[Category: Beta-trefoil]] | ||
[[Category: F42w/w120f double mutant]] | [[Category: F42w/w120f double mutant]] | ||
Revision as of 07:15, 25 December 2014
Crystal structure of Interleukin 1-beta F42W/W120F mutantCrystal structure of Interleukin 1-beta F42W/W120F mutant
Structural highlights
Function[IL1B_HUMAN] Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.[1] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe crystal structure at 1.54 A resolution of a double mutant of interleukin-1beta (F42W/W120F), a cytokine secreted by macrophages, was determined by multiple-wavelength anomalous dispersion (MAD) using data from highly twinned selenomethionine-modified crystals. The space group is P4(3), with unit-cell parameters a = b = 53.9, c = 77.4 A. Self-rotation function analysis and various intensity statistics revealed the presence of merohedral twinning in crystals of both the native (twinning fraction alpha approximately 0.35) and SeMet (alpha approximately 0.40) forms. Structure determination and refinement are discussed with emphasis on the possible reasons for successful phasing using untreated twinned MAD data. Use of multiple anomalous dispersion to phase highly merohedrally twinned crystals of interleukin-1beta.,Rudolph MG, Kelker MS, Schneider TR, Yeates TO, Oseroff V, Heidary DK, Jennings PA, Wilson IA Acta Crystallogr D Biol Crystallogr. 2003 Feb;59(Pt 2):290-8. Epub 2003, Jan 23. PMID:12554939[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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