1nyb: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1nyb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_phi21 Enterobacteria phage phi21]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NYB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NYB FirstGlance]. <br> | <table><tr><td colspan='2'>[[1nyb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_phi21 Enterobacteria phage phi21]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NYB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NYB FirstGlance]. <br> | ||
</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1a4t|1a4t]]</td></tr> | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1a4t|1a4t]]</td></tr> | ||
<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">N ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10737 Enterobacteria phage phi21])</td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">N ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10737 Enterobacteria phage phi21])</td></tr> | ||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nyb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nyb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1nyb RCSB], [http://www.ebi.ac.uk/pdbsum/1nyb PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nyb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nyb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1nyb RCSB], [http://www.ebi.ac.uk/pdbsum/1nyb PDBsum]</span></td></tr> | ||
<table> | </table> | ||
== Function == | |||
[[http://www.uniprot.org/uniprot/REGN_BPPH3 REGN_BPPH3]] N protein regulates the transition from the early to the middle stage of lytic development. | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Enterobacteria phage phi21]] | [[Category: Enterobacteria phage phi21]] | ||
[[Category: Cilley, C D | [[Category: Cilley, C D]] | ||
[[Category: Williamson, J R | [[Category: Williamson, J R]] | ||
[[Category: Peptide-rna complex]] | [[Category: Peptide-rna complex]] | ||
[[Category: Transcription antitermination]] | [[Category: Transcription antitermination]] | ||
[[Category: Transcription-rna complex]] | [[Category: Transcription-rna complex]] | ||
Revision as of 22:27, 25 December 2014
SOLUTION STRUCTURE OF THE BACTERIOPHAGE PHI21 N PEPTIDE-BOXB RNA COMPLEXSOLUTION STRUCTURE OF THE BACTERIOPHAGE PHI21 N PEPTIDE-BOXB RNA COMPLEX
Structural highlights
Function[REGN_BPPH3] N protein regulates the transition from the early to the middle stage of lytic development. Publication Abstract from PubMedWe determined the solution structure of a 22-amino-acid peptide from the amino-terminal domain of the bacteriophage phi21 N protein in complex with its cognate 24-mer boxB RNA hairpin using heteronuclear magnetic resonance spectroscopy. The N peptide binds as an alpha-helix and interacts predominately with the major groove side of the 5' half of the boxB RNA stem-loop. This binding interface is defined by surface complementarity of polar and nonpolar interactions, and little sequence-specific recognition. The phi21 boxB loop (CUAACC) has hydrogen bond and backbone torsions typical of the "U-turn" motif, as well as base stacking of the last 4 nt, and a hydrogen bonded C:C pair closing the loop. The exposed face of the phi21 boxB loop, in complex with the N peptide, is strikingly similar to the GNRA tetraloop-like folds of the related lambda and P22 bacteriophage N peptide-boxB RNA complexes. The N peptide-boxB complexes of the various phage, while individually distinct, provide similar structural features for interactions with the Escherichia coli host factors to enable antitermination. Structural mimicry in the phage phi21 N peptide-boxB RNA complex.,Cilley CD, Williamson JR RNA. 2003 Jun;9(6):663-76. PMID:12756325[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References |
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