1e9r: Difference between revisions
No edit summary |
No edit summary |
||
| Line 4: | Line 4: | ||
|PDB= 1e9r |SIZE=350|CAPTION= <scene name='initialview01'>1e9r</scene>, resolution 2.40Å | |PDB= 1e9r |SIZE=350|CAPTION= <scene name='initialview01'>1e9r</scene>, resolution 2.40Å | ||
|SITE= <scene name='pdbsite=SO1:So4+Binding+Site+For+Residue+G602+Nucleotide-Binding+Sit+...'>SO1</scene> | |SITE= <scene name='pdbsite=SO1:So4+Binding+Site+For+Residue+G602+Nucleotide-Binding+Sit+...'>SO1</scene> | ||
|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= TRWB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |GENE= TRWB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e9r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e9r OCA], [http://www.ebi.ac.uk/pdbsum/1e9r PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1e9r RCSB]</span> | |||
}} | }} | ||
| Line 27: | Line 30: | ||
[[Category: Gomis-Rueth, F X.]] | [[Category: Gomis-Rueth, F X.]] | ||
[[Category: Moncalian, G.]] | [[Category: Moncalian, G.]] | ||
[[Category: bacterial conjugation]] | [[Category: bacterial conjugation]] | ||
[[Category: coupling protein]] | [[Category: coupling protein]] | ||
| Line 33: | Line 35: | ||
[[Category: ring helicase]] | [[Category: ring helicase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:58:12 2008'' | ||
Revision as of 19:58, 30 March 2008
| |||||||
| , resolution 2.40Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | |||||||
| Gene: | TRWB (Escherichia coli) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
BACTERIAL CONJUGATIVE COUPLING PROTEIN TRWBDELTAN70. TRIGONAL FORM IN COMPLEX WITH SULPHATE.
OverviewOverview
The transfer of DNA across membranes and between cells is a central biological process; however, its molecular mechanism remains unknown. In prokaryotes, trans-membrane passage by bacterial conjugation, is the main route for horizontal gene transfer. It is the means for rapid acquisition of new genetic information, including antibiotic resistance by pathogens. Trans-kingdom gene transfer from bacteria to plants or fungi and even bacterial sporulation are special cases of conjugation. An integral membrane DNA-binding protein, called TrwB in the Escherichia coli R388 conjugative system, is essential for the conjugation process. This large multimeric protein is responsible for recruiting the relaxosome DNA-protein complex, and participates in the transfer of a single DNA strand during cell mating. Here we report the three-dimensional structure of a soluble variant of TrwB. The molecule consists of two domains: a nucleotide-binding domain of alpha/beta topology, reminiscent of RecA and DNA ring helicases, and an all-alpha domain. Six equivalent protein monomers associate to form an almost spherical quaternary structure that is strikingly similar to F1-ATPase. A central channel, 20 A in width, traverses the hexamer.
About this StructureAbout this Structure
1E9R is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
The bacterial conjugation protein TrwB resembles ring helicases and F1-ATPase., Gomis-Ruth FX, Moncalian G, Perez-Luque R, Gonzalez A, Cabezon E, de la Cruz F, Coll M, Nature. 2001 Feb 1;409(6820):637-41. PMID:11214325
Page seeded by OCA on Sun Mar 30 19:58:12 2008