1e7k: Difference between revisions
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|PDB= 1e7k |SIZE=350|CAPTION= <scene name='initialview01'>1e7k</scene>, resolution 2.9Å | |PDB= 1e7k |SIZE=350|CAPTION= <scene name='initialview01'>1e7k</scene>, resolution 2.9Å | ||
|SITE= | |SITE= | ||
|LIGAND= | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1e7k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e7k OCA], [http://www.ebi.ac.uk/pdbsum/1e7k PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1e7k RCSB]</span> | |||
}} | }} | ||
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[[Category: u4 snrna]] | [[Category: u4 snrna]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:56:50 2008'' | ||
Revision as of 19:56, 30 March 2008
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| , resolution 2.9Å | |||||||
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| Ligands: | , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF THE SPLICEOSOMAL 15.5KD PROTEIN BOUND TO A U4 SNRNA FRAGMENT
OverviewOverview
We have determined the crystal structure of a spliceosomal RNP complex comprising the 15.5kD protein of the human U4/U6.U5 tri-snRNP and the 5' stem-loop of U4 snRNA. The protein interacts almost exclusively with a purine-rich (5+2) internal loop within the 5' stem-loop, giving an unusual RNA fold characterized by two tandem sheared G-A base pairs, a high degree of purine stacking, and the accommodation of a single RNA base, rotated out of the RNA chain, in a pocket of the protein. Apart from yielding the structure of an important entity in the pre-mRNA splicing apparatus, this work also implies a model for the complex of the 15.5kD protein with box C/D snoRNAs. It additionally suggests a general recognition principle in a novel family of RNA binding proteins.
About this StructureAbout this Structure
1E7K is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of the spliceosomal 15.5kD protein bound to a U4 snRNA fragment., Vidovic I, Nottrott S, Hartmuth K, Luhrmann R, Ficner R, Mol Cell. 2000 Dec;6(6):1331-42. PMID:11163207
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