1d5t: Difference between revisions
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|PDB= 1d5t |SIZE=350|CAPTION= <scene name='initialview01'>1d5t</scene>, resolution 1.04Å | |PDB= 1d5t |SIZE=350|CAPTION= <scene name='initialview01'>1d5t</scene>, resolution 1.04Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1gnd|1gnd]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1d5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d5t OCA], [http://www.ebi.ac.uk/pdbsum/1d5t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1d5t RCSB]</span> | |||
}} | }} | ||
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[[Category: Wilson, I A.]] | [[Category: Wilson, I A.]] | ||
[[Category: Zeng, K.]] | [[Category: Zeng, K.]] | ||
[[Category: ultra-high resolution]] | [[Category: ultra-high resolution]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:35:19 2008'' | ||
Revision as of 19:35, 30 March 2008
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| , resolution 1.04Å | |||||||
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| Related: | 1gnd
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
GUANINE NUCLEOTIDE DISSOCIATION INHIBITOR, ALPHA-ISOFORM
OverviewOverview
Guanine nucleotide dissociation inhibitor (GDI) is a 55-kDa protein that functions in vesicular membrane transport to recycle Rab GTPases. We have now determined the crystal structure of bovine alpha-GDI at ultra-high resolution (1.04 A). Refinement at this resolution highlighted a region with high mobility of its main-chain residues. This corresponded to a surface loop in the primarily alpha-helical domain II at the base of alpha-GDI containing the previously uncharacterized sequence-conserved region (SCR) 3A. Site-directed mutagenesis showed that this mobile loop plays a crucial role in binding of GDI to membranes and extraction of membrane-bound Rab. This domain, referred to as the mobile effector loop, in combination with Rab-binding residues found in the multi-sheet domain I at the apex of alpha-GDI may provide flexibility for recycling of diverse Rab GTPases. We propose that conserved residues in domains I and II synergize to form the functional face of GDI, and that domain II mediates a critical step in Rab recycling during vesicle fusion.
About this StructureAbout this Structure
1D5T is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
ReferenceReference
A new functional domain of guanine nucleotide dissociation inhibitor (alpha-GDI) involved in Rab recycling., Luan P, Heine A, Zeng K, Moyer B, Greasely SE, Kuhn P, Balch WE, Wilson IA, Traffic. 2000 Mar;1(3):270-81. PMID:11208110
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