1cxk: Difference between revisions
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|PDB= 1cxk |SIZE=350|CAPTION= <scene name='initialview01'>1cxk</scene>, resolution 2.090Å | |PDB= 1cxk |SIZE=350|CAPTION= <scene name='initialview01'>1cxk</scene>, resolution 2.090Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:GLUCOSE'>GLC</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Cyclomaltodextrin_glucanotransferase Cyclomaltodextrin glucanotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.19 2.4.1.19] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cxk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cxk OCA], [http://www.ebi.ac.uk/pdbsum/1cxk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1cxk RCSB]</span> | |||
}} | }} | ||
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[[Category: Kalk, K H.]] | [[Category: Kalk, K H.]] | ||
[[Category: Uitdehaag, J C.M.]] | [[Category: Uitdehaag, J C.M.]] | ||
[[Category: alpha-amylase family]] | [[Category: alpha-amylase family]] | ||
[[Category: glycosyl hydrolase family 13]] | [[Category: glycosyl hydrolase family 13]] | ||
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[[Category: substrate complex]] | [[Category: substrate complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:30:31 2008'' | ||
Revision as of 19:30, 30 March 2008
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| , resolution 2.090Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Activity: | Cyclomaltodextrin glucanotransferase, with EC number 2.4.1.19 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
COMPLEX BETWEEN A MALTONONAOSE SUBSTRATE AND BACILLUS CIRCULANS STRAIN 251 CGTASE E257Q/D229N
OverviewOverview
Cyclodextrin glycosyltransferase (CGTase) is an enzyme of the alpha-amylase family, which uses a double displacement mechanism to process alpha-linked glucose polymers. We have determined two X-ray structures of CGTase complexes, one with an intact substrate at 2.1 A resolution, and the other with a covalently bound reaction intermediate at 1.8 A resolution. These structures give evidence for substrate distortion and the covalent character of the intermediate and for the first time show, in atomic detail, how catalysis in the alpha-amylase family proceeds by the concerted action of all active site residues.
About this StructureAbout this Structure
1CXK is a Single protein structure of sequence from Bacillus circulans. Full crystallographic information is available from OCA.
ReferenceReference
X-ray structures along the reaction pathway of cyclodextrin glycosyltransferase elucidate catalysis in the alpha-amylase family., Uitdehaag JC, Mosi R, Kalk KH, van der Veen BA, Dijkhuizen L, Withers SG, Dijkstra BW, Nat Struct Biol. 1999 May;6(5):432-6. PMID:10331869
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