1aj2: Difference between revisions
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|PDB= 1aj2 |SIZE=350|CAPTION= <scene name='initialview01'>1aj2</scene>, resolution 2.00Å | |PDB= 1aj2 |SIZE=350|CAPTION= <scene name='initialview01'>1aj2</scene>, resolution 2.00Å | ||
|SITE= | |SITE= | ||
|LIGAND= | |LIGAND= <scene name='pdbligand=2PH:[7,8-DIHYDRO-PTERIN-6-YL+METHANYL]-PHOSPHONOPHOSPHATE'>2PH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Dihydropteroate_synthase Dihydropteroate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.15 2.5.1.15] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydropteroate_synthase Dihydropteroate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.15 2.5.1.15] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1aj2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aj2 OCA], [http://www.ebi.ac.uk/pdbsum/1aj2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1aj2 RCSB]</span> | |||
}} | }} | ||
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[[Category: Somers, D O.]] | [[Category: Somers, D O.]] | ||
[[Category: Stammers, D K.]] | [[Category: Stammers, D K.]] | ||
[[Category: antibiotic]] | [[Category: antibiotic]] | ||
[[Category: biosynthesis]] | [[Category: biosynthesis]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:41:21 2008'' | ||
Revision as of 18:41, 30 March 2008
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| , resolution 2.00Å | |||||||
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| Ligands: | , | ||||||
| Activity: | Dihydropteroate synthase, with EC number 2.5.1.15 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF A BINARY COMPLEX OF E. COLI DIHYDROPTEROATE SYNTHASE
OverviewOverview
Sulfonamides were amongst the first clinically useful antibacterial agents to be discovered. The identification of sulfanilamide as the active component of the dye Prontosil rubrum led to the synthesis of clinically useful analogues. Today sulfamethoxazole (in combination with trimethoprim), is used to treat urinary tract infections caused by bacteria such as Escherichia coli and is also a first-line treatment for pneumonia caused by the fungus Pneumocystis carinii, a common condition in AIDS patients. The site of action is the de novo folate biosynthesis enzyme dihydropteroate synthase (DHPS) where sulfonamides act as analogues of one of the substrates, para-aminobenzoic acid (pABA). We report here the crystal structure of E.coli DHPS at 2.0 A resolution refined to an R-factor of 0.185. The single domain of 282 residues forms an eight-stranded alpha/beta-barrel. The 7,8-dihydropterin pyrophosphate (DHPPP) substrate binds in a deep cleft in the barrel, whilst sulfanilamide binds closer to the surface. The DHPPP ligand site is highly conserved amongst prokaryotic and eukaryotic DHPSs.
About this StructureAbout this Structure
1AJ2 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of the anti-bacterial sulfonamide drug target dihydropteroate synthase., Achari A, Somers DO, Champness JN, Bryant PK, Rosemond J, Stammers DK, Nat Struct Biol. 1997 Jun;4(6):490-7. PMID:9187658
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