Monoglyceride lipase: Difference between revisions
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===Catalytic Triad=== | ===Catalytic Triad=== | ||
MGL’s serine hydrolase chemistry is executed by a <scene name='57/573134/Catalytic_triad/4'>Catalytic Triad</scene> (Ser132-His279-Asp249) and seems to utilize the same mechanism as the much-studied [http://en.wikipedia.org/wiki/Chymotrypsin chymotrypsin]. In this mechanism, an activated serine nucleophile cleaves the ester bond of the substrate.<ref name="labar" /><ref name="bert" /><ref name="shalk" /> The subsequent tetrahedral intermediate is stabilized by the <scene name='57/573134/Oxyanion_hole/3'>Oxyanion Hole</scene>, formed by the main-chain nitrogens of Ala61 and Met (or Se-Met) 133.<ref name="bert" /> The triad was found using site-directed mutagenesis of each individual residue and each of these amino acid residues are catalytically essential to MGL <ref name=" | MGL’s serine hydrolase chemistry is executed by a <scene name='57/573134/Catalytic_triad/4'>Catalytic Triad</scene> (Ser132-His279-Asp249) and seems to utilize the same mechanism as the much-studied [http://en.wikipedia.org/wiki/Chymotrypsin chymotrypsin]. In this mechanism, an activated serine nucleophile cleaves the ester bond of the substrate.<ref name="labar" /><ref name="bert" /><ref name="shalk" /> The subsequent tetrahedral intermediate is stabilized by the <scene name='57/573134/Oxyanion_hole/3'>Oxyanion Hole</scene>, formed by the main-chain nitrogens of Ala61 and Met (or Se-Met) 133.<ref name="bert" /> The triad was found using site-directed mutagenesis of each individual residue and each of these amino acid residues are catalytically essential to MGL <ref name="bert"/>. The catalytic triad is located in the [[:Category:Ligand binding pocket| Binding Pocket]] buried at the bottom of it in the oxyanion hole connected by a water molecule. | ||
===Ligand Binding Site=== | ===Ligand Binding Site=== | ||