2npm: Difference between revisions
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<StructureSection load='2npm' size='340' side='right' caption='[[2npm]], [[Resolution|resolution]] 2.52Å' scene=''> | <StructureSection load='2npm' size='340' side='right' caption='[[2npm]], [[Resolution|resolution]] 2.52Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2npm]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cryptosporidium_parvum Cryptosporidium parvum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NPM OCA]. <br> | <table><tr><td colspan='2'>[[2npm]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cryptosporidium_parvum Cryptosporidium parvum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NPM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2NPM FirstGlance]. <br> | ||
</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr> | </td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr> | ||
<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cgd3_1290 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5807 Cryptosporidium parvum])</td></tr> | <tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cgd3_1290 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5807 Cryptosporidium parvum])</td></tr> | ||
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2npm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2npm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2npm RCSB], [http://www.ebi.ac.uk/pdbsum/2npm PDBsum]</span></td></tr> | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2npm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2npm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2npm RCSB], [http://www.ebi.ac.uk/pdbsum/2npm PDBsum]</span></td></tr> | ||
<table> | <table> | ||
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Characterization of 14-3-3 proteins from Cryptosporidium parvum.,Brokx SJ, Wernimont AK, Dong A, Wasney GA, Lin YH, Lew J, Vedadi M, Lee WH, Hui R PLoS One. 2011;6(8):e14827. Epub 2011 Aug 11. PMID:21853016<ref>PMID:21853016</ref> | Characterization of 14-3-3 proteins from Cryptosporidium parvum.,Brokx SJ, Wernimont AK, Dong A, Wasney GA, Lin YH, Lew J, Vedadi M, Lee WH, Hui R PLoS One. 2011;6(8):e14827. Epub 2011 Aug 11. PMID:21853016<ref>PMID:21853016</ref> | ||
From | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
== References == | == References == | ||
Revision as of 12:21, 3 October 2014
crystal structure of Cryptosporidium parvum 14-3-3 protein in complex with peptidecrystal structure of Cryptosporidium parvum 14-3-3 protein in complex with peptide
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe parasite Cryptosporidium parvum has three 14-3-3 proteins: Cp14epsilon, Cp14a and Cp14b, with only Cp14epsilon similar to human 14-3-3 proteins in sequence, peptide-binding properties and structure. Structurally, Cp14a features the classical 14-3-3 dimer but with a uniquely wide pocket and a disoriented RRY triad potentially incapable of binding phosphopeptides. The Cp14b protein deviates from the norm significantly: (i) In one subunit, the phosphorylated C-terminal tail is bound in the binding groove like a phosphopeptide. This supports our binding study indicating this protein was stabilized by a peptide mimicking its last six residues. (ii) The other subunit has eight helices instead of nine, with alphaA and alphaB forming a single helix and occluding the peptide-binding cleft. (iii) The protein forms a degenerate dimer with the two binding grooves divided and facing opposite directions. These features conspire to block and disrupt the bicameral substrate-binding pocket, suggesting a possible tripartite auto-regulation mechanism that has not been observed previously. ENHANCED VERSION: This article can also be viewed as an enhanced version in which the text of the article is integrated with interactive 3D representations and animated transitions. Please note that a web plugin is required to access this enhanced functionality. Instructions for the installation and use of the web plugin are available in Text S1. Characterization of 14-3-3 proteins from Cryptosporidium parvum.,Brokx SJ, Wernimont AK, Dong A, Wasney GA, Lin YH, Lew J, Vedadi M, Lee WH, Hui R PLoS One. 2011;6(8):e14827. Epub 2011 Aug 11. PMID:21853016[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Cryptosporidium parvum
- Arrowsmith, C H.
- Bochkarev, A.
- Brokx, S.
- Dong, A.
- Doyle, D.
- Edwards, A M.
- Hassanali, A.
- Hui, R.
- Koeieradzki, I.
- Lew, J.
- Lin, L.
- Qiu, W.
- Ren, H.
- SGC, Structural Genomics Consortium.
- Sundstrom, M.
- Vedadi, M.
- Wasney, G.
- Weigelt, J.
- Zhao, Y.
- Cell regulator protein 14-3-3
- Protein binding
- Sgc
- Structural genomic
- Structural genomics consortium