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Crystal structure of the earthworm lectin C-terminal domain mutant in complex with 6'-sialyllactose

OverviewOverview

Sialic acid (Sia) is a typical terminal sugar, which modifies various types of glycoconjugates commonly found in higher animals. Its regulatory roles in diverse biological phenomena are frequently triggered by interaction with Sia-binding lectins. When using natural Sia-binding lectins as probes, however, there have been practical problems concerning their repertoire and availability. Here, we show a rational creation of a Sia-binding lectin based on the strategy 'natural evolution-mimicry', where Sia-binding lectins are engineered by error-prone PCR from a Gal-binding lectin used as a scaffold protein. After selection with fetuin-agarose using a recently reinforced ribosome display system, one of the evolved mutants SRC showed substantial affinity for alpha2-6Sia, which the parental Gal-binding lectin EW29Ch lacked. SRC was found to have additional practical advantages in productivity and in preservation of affinity for Gal. Thus, the developed novel Sia-recognition protein will contribute as useful tools to sialoglycomics.

About this StructureAbout this Structure

2DS0 is a Single protein structure of sequence from Lumbricus terrestris. Full crystallographic information is available from OCA.

ReferenceReference

Tailoring a novel sialic acid-binding lectin from a ricin-B chain-like galactose-binding protein by natural evolution-mimicry., Yabe R, Suzuki R, Kuno A, Fujimoto Z, Jigami Y, Hirabayashi J, J Biochem. 2007 Mar;141(3):389-99. Epub 2007 Jan 18. PMID:17234683

Page seeded by OCA on Thu Mar 20 16:30:45 2008

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OCA

@@ Line 1: / Line 1: @@
-[[Image:2ds0.jpg|left|200px]]<br /><applet load="2ds0" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:2ds0.jpg|left|200px]]
-caption="2ds0, resolution 1.80&Aring;" />
-'''Crystal structure of the earthworm lectin C-terminal domain mutant in complex with 6'-sialyllactose'''<br />
+ {{Structure
+|PDB= 2ds0 |SIZE=350|CAPTION= <scene name='initialview01'>2ds0</scene>, resolution 1.80&Aring;
+|SITE= <scene name='pdbsite=AC1:Sia+Binding+Site+For+Residue+B+270'>AC1</scene>, <scene name='pdbsite=AC2:Gal+Binding+Site+For+Residue+B+271'>AC2</scene>, <scene name='pdbsite=AC3:Sia+Binding+Site+For+Residue+A+280'>AC3</scene>, <scene name='pdbsite=AC4:Gal+Binding+Site+For+Residue+A+281'>AC4</scene>, <scene name='pdbsite=AC5:So4+Binding+Site+For+Residue+A+1001'>AC5</scene>, <scene name='pdbsite=AC6:So4+Binding+Site+For+Residue+A+1002'>AC6</scene>, <scene name='pdbsite=AC7:So4+Binding+Site+For+Residue+A+1003'>AC7</scene>, <scene name='pdbsite=AC8:So4+Binding+Site+For+Residue+A+1004'>AC8</scene> and <scene name='pdbsite=AC9:So4+Binding+Site+For+Residue+B+1005'>AC9</scene>
+|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''Crystal structure of the earthworm lectin C-terminal domain mutant in complex with 6'-sialyllactose'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-DS0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Lumbricus_terrestris Lumbricus terrestris] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=AC1:Sia+Binding+Site+For+Residue+B+270'>AC1</scene>, <scene name='pdbsite=AC2:Gal+Binding+Site+For+Residue+B+271'>AC2</scene>, <scene name='pdbsite=AC3:Sia+Binding+Site+For+Residue+A+280'>AC3</scene>, <scene name='pdbsite=AC4:Gal+Binding+Site+For+Residue+A+281'>AC4</scene>, <scene name='pdbsite=AC5:So4+Binding+Site+For+Residue+A+1001'>AC5</scene>, <scene name='pdbsite=AC6:So4+Binding+Site+For+Residue+A+1002'>AC6</scene>, <scene name='pdbsite=AC7:So4+Binding+Site+For+Residue+A+1003'>AC7</scene>, <scene name='pdbsite=AC8:So4+Binding+Site+For+Residue+A+1004'>AC8</scene> and <scene name='pdbsite=AC9:So4+Binding+Site+For+Residue+B+1005'>AC9</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DS0 OCA].
+DS0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lumbricus_terrestris Lumbricus terrestris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DS0 OCA].
 ==Reference==
-Tailoring a novel sialic acid-binding lectin from a ricin-B chain-like galactose-binding protein by natural evolution-mimicry., Yabe R, Suzuki R, Kuno A, Fujimoto Z, Jigami Y, Hirabayashi J, J Biochem. 2007 Mar;141(3):389-99. Epub 2007 Jan 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17234683 17234683]
+Tailoring a novel sialic acid-binding lectin from a ricin-B chain-like galactose-binding protein by natural evolution-mimicry., Yabe R, Suzuki R, Kuno A, Fujimoto Z, Jigami Y, Hirabayashi J, J Biochem. 2007 Mar;141(3):389-99. Epub 2007 Jan 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17234683 17234683]
 [[Category: Lumbricus terrestris]]
 [[Category: Single protein]]
@@ Line 24: / Line 33: @@
 [[Category: sugar complex]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Mar 14 09:42:06 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 16:30:45 2008''