1lc4: Difference between revisions
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==Crystal Structure of Tobramycin Bound to the Eubacterial 16S rRNA A Site== | |||
<StructureSection load='1lc4' size='340' side='right' caption='[[1lc4]], [[Resolution|resolution]] 2.54Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1lc4]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LC4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1LC4 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TOY:TOBRAMYCIN'>TOY</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1j7t|1j7t]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lc4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lc4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1lc4 RCSB], [http://www.ebi.ac.uk/pdbsum/1lc4 PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Aminoglycoside antibiotics target the decoding aminoacyl site (A site) on the 16S ribosomal RNA and induce miscoding during translation. Here, we present the crystal structure, at 2.54 A resolution, of an RNA oligonucleotide containing the A site sequence complexed to the 4,6-disubstituted 2-deoxystreptamine aminoglycoside tobramycin. The three aminosugar rings making up tobramycin interact with the deep-groove atoms directly or via water molecules and stabilize a fully bulged-out conformation of adenines A(1492) and A(1493). The comparison between this structure and the one previously solved in the presence of paromomycin confirms the importance of the functional groups on the common neamine part of these two antibiotics for binding to RNA. Furthermore, the analysis of the present structure provides a molecular explanation to some of the resistance mechanisms that have spread among bacteria and rendered aminoglycoside antibiotics inefficient. | |||
Crystal structure of a complex between the aminoglycoside tobramycin and an oligonucleotide containing the ribosomal decoding a site.,Vicens Q, Westhof E Chem Biol. 2002 Jun;9(6):747-55. PMID:12079787<ref>PMID:12079787</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
[[Category: Vicens, Q | == References == | ||
[[Category: Westhof, E | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Vicens, Q]] | |||
[[Category: Westhof, E]] | |||
[[Category: Adenine bulge]] | [[Category: Adenine bulge]] | ||
[[Category: Antibiotic-rna complex]] | [[Category: Antibiotic-rna complex]] | ||
[[Category: Rna]] | [[Category: Rna]] | ||
Revision as of 09:01, 22 December 2014
Crystal Structure of Tobramycin Bound to the Eubacterial 16S rRNA A SiteCrystal Structure of Tobramycin Bound to the Eubacterial 16S rRNA A Site
Structural highlights
Publication Abstract from PubMedAminoglycoside antibiotics target the decoding aminoacyl site (A site) on the 16S ribosomal RNA and induce miscoding during translation. Here, we present the crystal structure, at 2.54 A resolution, of an RNA oligonucleotide containing the A site sequence complexed to the 4,6-disubstituted 2-deoxystreptamine aminoglycoside tobramycin. The three aminosugar rings making up tobramycin interact with the deep-groove atoms directly or via water molecules and stabilize a fully bulged-out conformation of adenines A(1492) and A(1493). The comparison between this structure and the one previously solved in the presence of paromomycin confirms the importance of the functional groups on the common neamine part of these two antibiotics for binding to RNA. Furthermore, the analysis of the present structure provides a molecular explanation to some of the resistance mechanisms that have spread among bacteria and rendered aminoglycoside antibiotics inefficient. Crystal structure of a complex between the aminoglycoside tobramycin and an oligonucleotide containing the ribosomal decoding a site.,Vicens Q, Westhof E Chem Biol. 2002 Jun;9(6):747-55. PMID:12079787[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References |
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