3ppq: Difference between revisions

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-{{STRUCTURE_3ppq|  PDB=3ppq  |  SCENE=  }}
+==Structures of the substrate-binding protein provide insights into the multiple compatible solutes binding specificities of Bacillus subtilis ABC transporter OpuC==
-===Structures of the substrate-binding protein provide insights into the multiple compatible solutes binding specificities of Bacillus subtilis ABC transporter OpuC===
+<StructureSection load='3ppq' size='340' side='right' caption='[[3ppq]], [[Resolution|resolution]] 1.91&Aring;' scene=''>
-{{ABSTRACT_PUBMED_21366542}}
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3ppq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PPQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PPQ FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CHT:CHOLINE+ION'>CHT</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ppn|3ppn]], [[3ppo|3ppo]], [[3ppp|3ppp]], [[3ppr|3ppr]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">opuCC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Bacillus globigii" Migula 1900])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ppq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ppq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ppq RCSB], [http://www.ebi.ac.uk/pdbsum/3ppq PDBsum]</span></td></tr>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/OPUCC_BACSU OPUCC_BACSU]] Member of a high affinity multicomponent binding-protein-dependent transport system for glycine betaine, carnitine, and choline.<ref>PMID:10216873</ref> <ref>PMID:8752321</ref>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The compatible solute ATP-binding cassette (ABC) transporters are indispensable for acquiring a variety of compatible solutes under osmotic stress in Bacillus subtilis. The substrate-binding protein OpuCC of the ABC transporter OpuC can recognize a broad spectrum of compatible solutes, compared to its 70% sequence-identical paralog OpuBC that can solely bind choline. To explore the structural basis of this difference of substrate specificity, we determined crystal structures of OpuCC in the apo-form and in complex with carnitine, glycine betaine, choline and ectoine, respectively. OpuCC is composed of two alpha/beta/alpha globular sandwich domains linked by two hinge regions, with substrate-binding pocket located at the inter-domain cleft. Upon substrate-binding, the two domains shift towards each other to trap the substrate. Comparative structural analysis revealed a plastic pocket that fits various compatible solutes, which attributes the multiple-substrate binding property to OpuCC. This plasticity is a gain-of-function via a single-residue mutation of Thr94 in OpuCC versus Asp96 in OpuBC.
-==Function==
+Structures of the substrate-binding protein provide insights into the multiple compatible solutes binding specificities of Bacillus subtilis ABC transporter OpuC.,Du Y, Shi WW, He YX, Yang YH, Zhou CZ, Chen Y Biochem J. 2011 Mar 3. PMID:21366542<ref>PMID:21366542</ref>
-[[http://www.uniprot.org/uniprot/OPUCC_BACSU OPUCC_BACSU]] Member of a high affinity multicomponent binding-protein-dependent transport system for glycine betaine, carnitine, and choline.<ref>PMID:10216873</ref> <ref>PMID:8752321</ref>
-==About this Structure==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[3ppq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PPQ OCA].
+</div>
 ==See Also==
 *[[ABC transporter|ABC transporter]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:021366542</ref><references group="xtra"/><references/>
+__TOC__
+</StructureSection>
 [[Category: Bacillus globigii migula 1900]]
-[[Category: Chen, Y.]]
+[[Category: Chen, Y]]
-[[Category: Du, Y.]]
+[[Category: Du, Y]]
-[[Category: He, Y X.]]
+[[Category: He, Y X]]
-[[Category: Shi, W W.]]
+[[Category: Shi, W W]]
-[[Category: Yang, Y H.]]
+[[Category: Yang, Y H]]
-[[Category: Zhou, C Z.]]
+[[Category: Zhou, C Z]]
 [[Category: Alpha-beta-alpha sandwich]]
 [[Category: Osmoprotectant]]
 [[Category: Transport protein]]