4lw0: Difference between revisions

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-{{STRUCTURE_4lw0|  PDB=4lw0  |  SCENE=  }}
+==Structure of the THF riboswitch bound to adenine==
-===Structure of the THF riboswitch bound to adenine===
+<StructureSection load='4lw0' size='340' side='right' caption='[[4lw0]], [[Resolution|resolution]] 1.89&Aring;' scene=''>
-{{ABSTRACT_PUBMED_24388757}}
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4lw0]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LW0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4LW0 FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADE:ADENINE'>ADE</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3sd3|3sd3]], [[4lvv|4lvv]], [[4lvw|4lvw]], [[4lvy|4lvy]], [[4lvx|4lvx]], [[4lvz|4lvz]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lw0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4lw0 RCSB], [http://www.ebi.ac.uk/pdbsum/4lw0 PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The tetrahydrofolate (THF) riboswitch regulates folate transport and metabolism in a number of Firmicutes by cooperatively binding two molecules of THF. To further understand this riboswitch's specificity for THF, binding and regulatory activity of a series of THF analogs and antifolates were examined. Our data reveal that although binding is dominated by the RNA's interactions with the pterin moiety, the para-aminobenzoic acid (pABA) moiety plays a significant role in transcriptional regulation. Further, we find that adenine and several other analogs bind with high affinity by an alternative binding mechanism. Despite a similar affinity to THF, adenine is a poor regulator of transcriptional attenuation. These results demonstrate that binding alone does not determine a compound's effectiveness in regulating the activity of the riboswitch-a complication in current efforts to develop antimicrobials that target these RNAs.
-==About this Structure==
+A Disconnect between High-Affinity Binding and Efficient Regulation by Antifolates and Purines in the Tetrahydrofolate Riboswitch.,Trausch JJ, Batey RT Chem Biol. 2014 Feb 20;21(2):205-16. doi: 10.1016/j.chembiol.2013.11.012. Epub, 2014 Jan 2. PMID:24388757<ref>PMID:24388757</ref>
-[[4lw0]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4LW0 OCA].
-==Reference==
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-<ref group="xtra">PMID:024388757</ref><references group="xtra"/><references/>
+</div>
-[[Category: Batey, R T.]]
+== References ==
-[[Category: Trausch, J J.]]
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Batey, R T]]
+[[Category: Trausch, J J]]
 [[Category: Adenine binding]]
 [[Category: Aptamer]]