3bgw: Difference between revisions
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[[Image:3bgw.jpg|left|200px]] | [[Image:3bgw.jpg|left|200px]] | ||
'''The Structure Of A DnaB-Like Replicative Helicase And Its Interactions With Primase''' | {{Structure | ||
|PDB= 3bgw |SIZE=350|CAPTION= <scene name='initialview01'>3bgw</scene>, resolution 3.91Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= G40P ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=194966 Enterobacteria phage SP6]) | |||
}} | |||
'''The Structure Of A DnaB-Like Replicative Helicase And Its Interactions With Primase''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
3BGW is a [ | 3BGW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_sp6 Enterobacteria phage sp6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BGW OCA]. | ||
==Reference== | ==Reference== | ||
The structure of a DnaB-family replicative helicase and its interactions with primase., Wang G, Klein MG, Tokonzaba E, Zhang Y, Holden LG, Chen XS, Nat Struct Mol Biol. 2008 Jan;15(1):94-100. Epub 2007 Dec 23. PMID:[http:// | The structure of a DnaB-family replicative helicase and its interactions with primase., Wang G, Klein MG, Tokonzaba E, Zhang Y, Holden LG, Chen XS, Nat Struct Mol Biol. 2008 Jan;15(1):94-100. Epub 2007 Dec 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18157148 18157148] | ||
[[Category: Enterobacteria phage sp6]] | [[Category: Enterobacteria phage sp6]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: replication]] | [[Category: replication]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:58:51 2008'' | ||
Revision as of 19:58, 20 March 2008
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| , resolution 3.91Å | |||||||
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| Gene: | G40P (Enterobacteria phage SP6) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The Structure Of A DnaB-Like Replicative Helicase And Its Interactions With Primase
OverviewOverview
Helicases are essential enzymes for DNA replication, a fundamental process in all living organisms. The DnaB family are hexameric replicative helicases that unwind duplex DNA and coordinate with RNA primase and other proteins at the replication fork in prokaryotes. Here, we report the full-length crystal structure of G40P, a DnaB family helicase. The hexamer complex reveals an unusual architectural feature and a new type of assembly mechanism. The hexamer has two tiers: a three-fold symmetric N-terminal tier and a six-fold symmetric C-terminal tier. Monomers with two different conformations, termed cis and trans, come together to provide a topological solution for the dual symmetry within a hexamer. Structure-guided mutational studies indicate an important role for the N-terminal tier in binding primase and regulating primase-mediated stimulation of helicase activity. This study provides insights into the structural and functional interplay between G40P helicase and DnaG primase.
About this StructureAbout this Structure
3BGW is a Single protein structure of sequence from Enterobacteria phage sp6. Full crystallographic information is available from OCA.
ReferenceReference
The structure of a DnaB-family replicative helicase and its interactions with primase., Wang G, Klein MG, Tokonzaba E, Zhang Y, Holden LG, Chen XS, Nat Struct Mol Biol. 2008 Jan;15(1):94-100. Epub 2007 Dec 23. PMID:18157148
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