Sandbox Reserved 818: Difference between revisions
Paul Giroud (talk | contribs) No edit summary |
Paul Giroud (talk | contribs) No edit summary |
||
| Line 32: | Line 32: | ||
==Toxin entry and trafficking in target cells== | ==Toxin entry and trafficking in target cells== | ||
After binding to the target cell receptors, PTX enters the cells via receptor mediated endocytosis, and then follows the retrograde transport system, involving both the Golgi apparatus and the endoplasmic reticulum [75,76]. | After binding to the target cell receptors, PTX enters the cells via receptor mediated endocytosis, and then follows the retrograde transport system, involving both the Golgi apparatus and the endoplasmic reticulum | ||
<ref name="Schmidt MA (1997)"> | |||
El Baya, A., Linnemann, R., von Olleschik-Elbheim, L., Robenek, H., & Schmidt, M. A. (1997). Endocytosis and retrograde transport of pertussis toxin to the Golgi complex as a prerequisite for cellular intoxication. European journal of cell biology, 73(1), 40. | |||
</ref>.[75,76]. | |||
Electron microscopy studies have shown that PTX enters the cells via coated pits | Electron microscopy studies have shown that PTX enters the cells via coated pits | ||
<ref name="Schmidt MA (1997)"> | |||
El Baya, A., Linnemann, R., von Olleschik-Elbheim, L., Robenek, H., & Schmidt, M. A. (1997). Endocytosis and retrograde transport of pertussis toxin to the Golgi complex as a prerequisite for cellular intoxication. European journal of cell biology, 73(1), 40. | |||
</ref>. | |||
But for now, PTX does not contain a clearly identified translocation domain in the B moiety. | |||
S1 is able to bind to phospholipids bilayers [78], suggesting that it may directly interact with the target cell membranes and mediate its translocation, and also that the B oligomer is not essential for this step. Results obtained from cell transfection experiments support this hypothesis [80,81]. | S1 is able to bind to phospholipids bilayers [78], suggesting that it may directly interact with the target cell membranes and mediate its translocation, and also that the B oligomer is not essential for this step. Results obtained from cell transfection experiments support this hypothesis [80,81]. | ||
| Line 67: | Line 74: | ||
==Reference== | ==Reference== | ||
<references/> | |||
<ref group="xtra">PMID:008075982</ref><references group="xtra"/> | <ref group="xtra">PMID:008075982</ref><references group="xtra"/> | ||
[[Category: Bordetella pertussis]] | [[Category: Bordetella pertussis]] | ||