3win: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Clostridium botulinum Hemagglutinin== | |||
===Clostridium botulinum | <StructureSection load='3win' size='340' side='right' caption='[[3win]], [[Resolution|resolution]] 3.50Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3win]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_botulinum_b Clostridium botulinum b]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WIN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WIN FirstGlance]. <br> | |||
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ha3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36827 Clostridium botulinum B]), hem17/B, ha2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36827 Clostridium botulinum B]), ha1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36827 Clostridium botulinum B])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3win FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3win OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3win RCSB], [http://www.ebi.ac.uk/pdbsum/3win PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Clostridium botulinum HA is a component of the large botulinum neurotoxin complex and is critical for its oral toxicity. HA plays multiple roles in toxin penetration in the gastrointestinal tract, including protection from the digestive environment, binding to the intestinal mucosal surface, and disruption of the epithelial barrier. At least two properties of HA contribute to these roles: the sugar-binding activity and the barrier-disrupting activity that depends on E-cadherin binding of HA. HA consists of three different proteins, HA1, HA2, and HA3, whose structures have been partially solved and are made up mainly of beta-strands. Here, we demonstrate structural and functional reconstitution of whole HA and present the complete structure of HA of serotype B determined by x-ray crystallography at 3.5 A resolution. This structure reveals whole HA to be a huge triskelion-shaped molecule. Our results suggest that whole HA is functionally and structurally separable into two parts: HA1, involved in recognition of cell-surface carbohydrates, and HA2-HA3, involved in paracellular barrier disruption by E-cadherin binding. | |||
Crystal Structure of Clostridium botulinum Whole Hemagglutinin Reveals a Huge Triskelion-shaped Molecular Complex.,Amatsu S, Sugawara Y, Matsumura T, Kitadokoro K, Fujinaga Y J Biol Chem. 2013 Dec 6;288(49):35617-25. doi: 10.1074/jbc.M113.521179. Epub 2013, Oct 28. PMID:24165130<ref>PMID:24165130</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Clostridium botulinum b]] | [[Category: Clostridium botulinum b]] | ||
[[Category: Amatsu, S | [[Category: Amatsu, S]] | ||
[[Category: Fujinaga, Y | [[Category: Fujinaga, Y]] | ||
[[Category: Kitadokoro, K | [[Category: Kitadokoro, K]] | ||
[[Category: Matsumura, T | [[Category: Matsumura, T]] | ||
[[Category: Sugawara, Y | [[Category: Sugawara, Y]] | ||
[[Category: Bacterial pathogenesis]] | [[Category: Bacterial pathogenesis]] | ||
[[Category: Bacterial toxin]] | [[Category: Bacterial toxin]] | ||